Antibody:	anti-Nanog (ab21603)
Detection system:	Alexa Fluor 488
Assay notes:	Three anti-Nanog antibodies were mentioned in the paper. It is not specified which one was used.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Non-Uniform	1A upper merge , 1A upper Nanog	Expressed in epiblast progenitors (53% of all inner cell mass cells).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Uniform	1A lower Nanog , 1A lower merge	Expressed in all cells.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		4A Nanog , 4A merge	Expressed in epiblast progenitors. Expressed in more cells compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5A upper Nanog , 5A upper merge	Expressed in epiblast progenitors.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5A lower merge , 5A lower Nanog

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		S2B Nanog protein , S2B merge	Expressed in epiblast progenitors. Nanog RNAs and proteins colocalize to the same cells, and their low, high levels are correlated as well as their absence of expression.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present			Expressed in more cells compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present	Uniform		Expressed in all cells.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Non-Uniform		Expressed in epiblast progenitors (53% of all inner cell mass cells).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Non-Uniform		Expressed in more cells compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Uniform		Expressed in all cells.