Probe:	Clu probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Punctate	2A
TS22: hypothalamus	Present	Punctate	2A	Punctiform staining was noted in the anterior and lateral hypothalamic area.
TS22: brain ventricle	Present	Diffuse	2A	Expression was detected in the ependyma.
TS22: choroid plexus	Present	Diffuse	2A	Expression was detected in the ependyma.

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present	Punctate	2B	A laminar pattern of staining was seen in the neocortex.
TS24: brain ventricle	Strong	Regionally restricted	2B	Expression was detected in the ependyma.
TS24: choroid plexus	Strong	Regionally restricted	2B	Expression was detected in the ependyma.

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present	Punctate	2C	A laminar pattern of staining was seen in the neocortex.

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Weak			Few labeled astrocytes were detected.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present		2D	Astrocyte populations were clearly visualized through the hippocampus.
TS26: brain	Weak	Widespread	2D	Most neurons in the brain express very low but significant levels of staining.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present			Most neurons in the spinal cord express very low but significant levels of staining.

Structure	Level	Pattern	Image	Note
TS27: brain	Present		2E	The pattern of expressing cells from earlier timepoints remained visible in newborns.

Structure	Level	Pattern	Image	Note
TS27: brain	Present			The pattern of expressing cells from earlier timepoints remained visible at postnatal age 3.
TS27: spinal cord	Present			The pattern of expressing cells from earlier timepoints remained visible at postnatal age 3.

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Strong		2F	Strongly labeled round-shaped neuronal cells were detected through the facial nucleus.

Structure	Level	Pattern	Image	Note
TS28: dorsal motor nucleus of vagus nerve	Moderate			Dense populations of neurons were labeled.
TS28: trigeminal V mesencephalic nucleus	Moderate			Dense populations of neurons were labeled.
TS28: spinal cord ventral horn	Present
TS28: dorsal raphe nucleus	Moderate
TS28: lateral reticular nucleus	Moderate
TS28: locus coeruleus	Moderate
TS28: median raphe nucleus	Moderate
TS28: red nucleus	Moderate
TS28: solitary tract nucleus	Moderate
TS28: spinal cord dorsal nucleus	Moderate
TS28: spinal cord intermediate grey	Moderate
TS28: trigeminal V spinal sensory nucleus	Moderate

Structure	Level	Pattern	Image	Note
TS28: hypoglossal nucleus	Strong		2G	Strongly labeled round-shaped neuronal cells were detected through the hypoglossal nucleus.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2H	The pattern of expressing cells from earlier timepoints remained visible in newborns.

Structure	Level	Pattern	Image	Note
TS28: trigeminal V motor nucleus	Moderate		2I	Moderate to high levels of labeling were detected in neurons of the trigeminal motor nucleus.

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Present		3A	Many large roundshaped or multipolar cells, presumed to be mainly motoneurons, were visualized in the facial nucleus.

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Present		3B	Many large roundshaped or multipolar cells, presumed to be mainly motoneurons, were visualized in the facial nucleus.

Structure	Level	Pattern	Image	Note
TS28: hypoglossal nucleus	Present		3C	Many large roundshaped or multipolar cells, presumed to be mainly motoneurons, were visualized in the hypoglossal nucleus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3D	Numerous astrocytes expressing Clusterin mRNA were observed.
TS28: neocortex	Present		3D	Numerous astrocytes expressing Clusterin mRNA were observed.

Structure	Level	Pattern	Image	Note
TS28: brain nucleus	Present		3E

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present	Regionally restricted	3F	Many large roundshaped or multipolar cells, presumed to be mainly motoneurons, were visualized in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS28: trigeminal V motor nucleus	Strong		4A	Expression was detected in neurons.

Structure	Level	Pattern	Image	Note
TS28: trigeminal V mesencephalic nucleus	Strong		4B	Expression was detected in neurons.

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Strong		4C	Expression was detected in neurons.

Structure	Level	Pattern	Image	Note
TS28: dorsal motor nucleus of vagus nerve	Strong		4D	Expression was detected in neurons.
TS28: hypoglossal nucleus	Strong		4D	Expression was detected in neurons.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Strong	Regionally restricted	4E	Expression was strong in ventral horn neurons.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Strong	Regionally restricted	4F	Expression was strong in ventral horn neurons.

Structure	Level	Pattern	Image	Note
TS28: hypoglossal nucleus	Present		7K