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Gene Expression Data
Assay Details
Assay
Reference: J:227617 Garrett AM, et al., Analysis of Expression Pattern and Genetic Deletion of Netrin5 in the Developing Mouse. Front Mol Neurosci. 2016;9:3
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6279959
Gene symbol: Isl2
Gene name: insulin related protein 2 (islet 2)
Antibody: clone 39.4D5
Detection system: Alexa Fluor 488
Results
Specimen 2E: embryonic day 13.5 (more )
Note: Triple labeled: green - Isl1 and Isl2; red - Egr2 (assay MGI:6279948); blue - DAPI.
Structure Level Pattern Image Note
TS21: spinal component of peripheral nervous system Ambiguous 2E
Specimen 2F: embryonic day 13.5; Ntn5tm1.1Rwb/Ntn5tm1.1Rwb (more )
Note: Triple labeled: green - Isl1 and Isl2; red - Egr2 (assay MGI:6279948); blue - DAPI.
Structure Level Pattern Image Note
TS21: spinal component of peripheral nervous system Ambiguous 2F
Specimen 2H: embryonic day 13.5 (more )
Note: Triple labeled: green - Isl1 and Isl2; red - Egr2 (assay MGI:6279948); blue - DAPI.
Structure Level Pattern Image Note
TS21: dorsal root ganglion Present Regionally restricted 2H Expression in motor neurons.
TS21: spinal cord Present Regionally restricted 2H Expression in motor neurons.
Specimen 2I: embryonic day 13.5; Ntn5tm1.1Rwb/Ntn5tm1.1Rwb (more )
Note: Triple labeled: green - Isl1 and Isl2; red - Egr2 (assay MGI:6279948); blue - DAPI.
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted 2I Expression in motor neurons.
Specimen 3A: embryonic day 13.5 (more )
Note: Double labeled: green - Isl1 and Isl2; red - neurofilament.
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted 3A Expression in motor neurons.
Specimen 3B: embryonic day 13.5; Ntn5tm1.1Rwb/Ntn5tm1.1Rwb (more )
Note: Double labeled: green - Isl1 and Isl2; red - neurofilament.
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted 3B Authors report expression in ectopic motor neuron cells bodies that had migrated out of the ventral horn and into ventral roots.
TS21: spinal nerve Present Regionally restricted 3B Authors report expression in ectopic motor neuron cells bodies that had migrated out of the ventral horn and into ventral roots.
Specimen 4A: embryonic day 13.5 (more )
Note: Double labeled: green - Isl1 and Isl2; red - neurofilament.
Structure Level Pattern Image Note
TS21: spinal nerve Absent 4A
Specimen 4B: embryonic day 13.5; Dscamdel17/Dscamdel17 (more )
Note: Double labeled: green - Isl1 and Isl2; red - neurofilament.
Structure Level Pattern Image Note
TS21: spinal nerve Ambiguous 4B Authors report ectopias occurred very rarely in Dscam mutant embryos.
Specimen 4D: embryonic day 13.5; Neo1Gt(KST265)Byg/Neo1Gt(KST265)Byg (more )
Note: Double labeled: green - Isl1 and Isl2; red - neurofilament.
Structure Level Pattern Image Note
TS21: spinal nerve Absent 4D Authors report no ectopias were observed in neogenin mutant embryos.
Specimen 4E: embryonic day 13.5; Dcctm1Wbg/Dcctm1Wbg (more )
Note: Double labeled: green - Isl1 and Isl2; red - neurofilament.
Structure Level Pattern Image Note
TS21: spinal nerve Present Regionally restricted 4E Authors report expression in ectopic motor neurons cell bodies located in rostral ventral roots of Dcc mutant embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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