Ntn5^tm1.1Rwb
Targeted Allele Detail

Summary

• Symbol: Ntn5^tm1.1Rwb
• Name: netrin 5; targeted mutation 1.1, Robert W Burgess
• MGI ID: MGI:5701693
• Gene: Ntn5 Location: Chr7:45333519-45343980 bp, + strand Genetic Position: Chr7, 29.43 cM
• Alliance: Ntn5^tm1.1Rwb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227617
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: Exons 2-6 were replaced with a cassette encoding a farnysylated YFP fused to the 5-prime sequence of exon2 and an FRT-flanked neomycin resistance cassette, and subsequent flpe-mediated excision deleted the neomycin selection cassette. Although sequencing confirmed that the integrated YFPF is in-frame with the start site, no expression was detected by either endogenous fluorescence or antibody staining. Although this deletion is also in intron 1 of Sec1, Q-RT-PCR did not detect any reduction in Sec1 expression. (J:227617)
• Inheritance: Not Specified

Expression

In Mice Carrying this Mutation:

41 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ntn5 Mutation: 14 strains or lines available

References

• Original: J:227617 Garrett AM, et al., Analysis of Expression Pattern and Genetic Deletion of Netrin5 in the Developing Mouse. Front Mol Neurosci. 2016;9:3
• All: 1 reference(s)