Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:29484
Bennett JH, et al., Bone morphogenetic protein-2 and -4 expression during murine orofacial development. Arch Oral Biol. 1995 Sep;40(9):847-54
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6277149
|
Gene symbol:
|
Bmp2
|
Gene name:
|
bone morphogenetic protein 2
|
Probe:
|
BMP2 cDNA
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
E9.5 not shown:
embryonic day 9.5
(more )
Specimen
E9.5 not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E10.5 not shown:
embryonic day 10.5
(more )
Specimen
E10.5 not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E11.5 not shown:
embryonic day 11.5
(more )
Specimen
E11.5 not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: embryo |
Present |
|
|
Authors state changes seen at E10.5 are still present at E11.5.
|
Specimen
1A:
embryonic day 13.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: tooth epithelium |
Present |
Regionally restricted |
1A
|
Expression in epithelium of ventral surface of tongue.
|
TS21: oral region |
Present |
Regionally restricted |
1A
|
Expression in floor of mouth.
|
Specimen
1B:
embryonic day 13.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
1C:
embryonic day 13.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
1D:
embryonic day 13.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: tongue epithelium |
Present |
Regionally restricted |
1D
|
Authors report a sharp cut-off between strong expression in the epithelium of the ventral surface of tongue and no expression in the dorsum.
|
Specimen
2A:
embryonic day 13.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: lower jaw tooth dental lamina |
Present |
|
2A
|
Expression in thickened dental lamina a earliest morphologically recognizable stage of mandibular tooth development.
|
TS21: tooth epithelium |
Present |
Regionally restricted |
2A
|
Expression in enamel epithelium.
|
Specimen
2B:
embryonic day 13.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: upper jaw tooth dental lamina |
Weak |
|
2B
|
Lesser extent of expression in dental lamina of maxillary tooth at slightly later stage of development.
|
TS21: upper jaw tooth mesenchyme |
Present |
Regionally restricted |
2B
|
No expression in underlying ectomesenchymal condensation, which will form dental follicle and papilla. Strong expression in surrounding ectomesenchyme.
|
Specimen
2C:
embryonic day 13.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|