Probe:	Nodal probe31
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present			Authors report expression from late morula stage.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5J	Expression in inner cell mass (ICM) cells of early to hatched blastocysts.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		5K	Expression in inner cell mass (ICM) cells of early to hatched blastocysts.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		5L	Expression in inner cell mass (ICM) cells of early to hatched blastocysts.

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		5M	Authors report majority of embryos with expression in both ICM derivatives.
TS6: primitive endoderm	Strong		5M	Authors report majority of embryos with expression in both ICM derivatives. In 61/86 embryos, primitive endoderm (PrE) expression was stronger in some cells than in others.

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		5N	In 25/86 embryos, expression exclusively in epiblast.

Structure	Level	Pattern	Image	Note
TS7: epiblast	Present		5O	Expression exclusively in epiblast.
TS7: primitive endoderm	Absent		5O	Expression exclusively in epiblast.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present		5P
TS8: primitive endoderm	Present		5P	Expression detected again in visceral endoderm at E5.25.