Antibody:	CV-2 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin

Structure	Level	Pattern	Image	Note
TS20: L4 vertebral cartilage condensation	Present	Regionally restricted	3F , 3D	Expression in prospective vertebral bodies.
TS20: L5 vertebral cartilage condensation	Present	Regionally restricted	3F , 3D	Expression in prospective vertebral bodies.

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Regionally restricted	4A	Expression in prospective vertebral bodies.

Structure	Level	Pattern	Image	Note
TS20: C1 vertebral cartilage condensation	Present	Regionally restricted	4B	Expression in prospective vertebral bodies of cervical vertebra C1 through C6.
TS20: C2 vertebral cartilage condensation	Present	Regionally restricted	4B	Expression in prospective vertebral bodies of cervical vertebra C1 through C6.
TS20: C3 vertebral cartilage condensation	Present	Regionally restricted	4B	Expression in prospective vertebral bodies of cervical vertebra C1 through C6.
TS20: C4 vertebral cartilage condensation	Present	Regionally restricted	4B	Expression in prospective vertebral bodies of cervical vertebra C1 through C6.
TS20: C5 vertebral cartilage condensation	Present	Regionally restricted	4B	Expression in prospective vertebral bodies of cervical vertebra C1 through C6.
TS20: C6 vertebral cartilage condensation	Present	Regionally restricted	4B	Expression in prospective vertebral bodies of cervical vertebra C1 through C6.

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Regionally restricted	4C	Authors state expression is normal in Chd-/- mutant embryos.
TS20: chondrocranium bone	Present	Regionally restricted	4C	Author report expression in basioccipital bone anterior to vertebral column.

Structure	Level	Pattern	Image	Note
TS20: C1 vertebral cartilage condensation	Present	Regionally restricted	4D	Authors state expression is similar to that of wild-type embryos.
TS20: C2 vertebral cartilage condensation	Present	Regionally restricted	4D	Authors state expression is similar to that of wild-type embryos.
TS20: C3 vertebral cartilage condensation	Present	Regionally restricted	4D	Authors state expression is similar to that of wild-type embryos.
TS20: C4 vertebral cartilage condensation	Present	Regionally restricted	4D	Authors state expression is similar to that of wild-type embryos.
TS20: C5 vertebral cartilage condensation	Present	Regionally restricted	4D	Authors state expression is similar to that of wild-type embryos.
TS20: C6 vertebral cartilage condensation	Present	Regionally restricted	4D	Authors state expression is similar to that of wild-type embryos.

Structure	Level	Pattern	Image	Note
TS20: embryo	Absent		4E

Structure	Level	Pattern	Image	Note
TS20: axial skeleton cervical region	Absent		4F

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Regionally restricted	S2C , S2A	Expression in prospective vertebral bodies.

Structure	Level	Pattern	Image	Note
TS20: embryo	Absent		S2D , S2B

Structure	Level	Pattern	Image	Note
TS20: C1 vertebral cartilage condensation	Present	Regionally restricted	S2G , S2E	Expression in prospective vertebral bodies.
TS20: C2 vertebral cartilage condensation	Present	Regionally restricted	S2G , S2E	Expression in prospective vertebral bodies.
TS20: C3 vertebral cartilage condensation	Present	Regionally restricted	S2G , S2E	Expression in prospective vertebral bodies.
TS20: C4 vertebral cartilage condensation	Present	Regionally restricted	S2G , S2E	Expression in prospective vertebral bodies.
TS20: C5 vertebral cartilage condensation	Present	Regionally restricted	S2G , S2E	Expression in prospective vertebral bodies.
TS20: C6 vertebral cartilage condensation	Present	Regionally restricted	S2G , S2E	Expression in prospective vertebral bodies.

Structure	Level	Pattern	Image	Note
TS20: C2 vertebral cartilage condensation	Present	Regionally restricted	S2H , S2F	Expression in region of lower tissue density corresponding to prospective vertebral bodies. Authors report expression in extracellular matrix, rather than on the surface of vertebral body cells.
TS20: C3 vertebral cartilage condensation	Present	Regionally restricted	S2H , S2F	Expression in region of lower tissue density corresponding to prospective vertebral bodies. Authors report expression in extracellular matrix, rather than on the surface of vertebral body cells.
TS20: C4 vertebral cartilage condensation	Present	Regionally restricted	S2H , S2F	Expression in region of lower tissue density corresponding to prospective vertebral bodies. Authors report expression in extracellular matrix, rather than on the surface of vertebral body cells.
TS20: C5 vertebral cartilage condensation	Present	Regionally restricted	S2H , S2F	Expression in region of lower tissue density corresponding to prospective vertebral bodies. Authors report expression in extracellular matrix, rather than on the surface of vertebral body cells.