Antibody:	Anti-Chordin AF758
Detection system:	Biotinylated secondary antibody/[Strept]avidin

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Regionally restricted	1C	Expression in prospective vertebral bodies. Expression predominantly in posterior half

Structure	Level	Pattern	Image	Note
TS20: embryo	Absent		1D

Structure	Level	Pattern	Image	Note
TS20: C3 vertebral cartilage condensation	Present	Regionally restricted	1F , 1E	Expression in vertebral bodies of cervical vertebra C2 through C4. Authors report expression in extracellular matrix of developing vertebral bodies.
TS20: C4 vertebral cartilage condensation	Present	Regionally restricted	1F , 1E	Expression in vertebral bodies of cervical vertebra C2 through C4. Authors report expression in extracellular matrix of developing vertebral bodies.

Structure	Level	Pattern	Image	Note
TS20: L4 vertebral cartilage condensation	Present	Regionally restricted	3C , 3A	At lumbar level, expression predominantly in posterior half of each prosprective vertebral body.
TS20: L5 vertebral cartilage condensation	Present	Regionally restricted	3C , 3A	At lumbar level, expression predominantly in posterior half of each prosprective vertebral body.

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Regionally restricted	5A	Expression in prospective vertebral bodies.

Structure	Level	Pattern	Image	Note
TS20: T3 vertebral cartilage condensation	Present	Regionally restricted	5B	Expression in prospective vertebral bodies. Preferential posterior expression not as marked as at lumbar levels. Authors report expression in extracellular matrix of developing vertebral bodies.
TS20: T4 vertebral cartilage condensation	Present	Regionally restricted	5B	Expression in prospective vertebral bodies. Preferential posterior expression not as marked as at lumbar levels. Authors report expression in extracellular matrix of developing vertebral bodies.

Structure	Level	Pattern	Image	Note
TS20: thoracic vertebral cartilage condensation	Absent		5C	Authors report no expression in vertebral bodies in the absence of CV2.

Structure	Level	Pattern	Image	Note
TS20: T3 vertebral cartilage condensation	Absent		5D	Authors report no expression in vertebral bodies in the absence of CV2.
TS20: T4 vertebral cartilage condensation	Absent		5D	Authors report no expression in vertebral bodies in the absence of CV2.
TS20: axial skeleton thoracic region	Strong	Regionally restricted	5D	Expression in intervertebral discs, at levels higher than seen in wild-type embryo.