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Gene Expression Data
Assay Details
Assay
Reference: J:163559 Messerschmidt DM, et al., Nanog is required for primitive endoderm formation through a non-cell autonomous mechanism. Dev Biol. 2010 Aug 1;344(1):129-37
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6272731
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog
Detection system: Alexa Fluor 488
Results
Specimen S1C control: embryonic day 3.5 (more )
Note: Double labeled: green - Nanog; blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Present S1C control
Specimen S1C gt1/gt1: embryonic day 3.5; NanogGt(D042B07)Wrst/NanogGt(D042B07)Wrst (more )
Note: Double labeled: green - Nanog; blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Absent S1C gt1/gt1
Specimen S1C gt2/gt2: embryonic day 3.5; NanogGt(D047A12)Wrst/NanogGt(D047A12)Wrst (more )
Note: Double labeled: green - Nanog; blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Absent S1C gt2/gt2
Specimen 1A/C: embryonic day 3.5; NanogGt(D042B07)Wrst/NanogGt(D047A12)Wrst (more )
Note: Triple labeled: green - Nanog; red - Cdx2 (assay MGI:6272733); blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Absent 1A , 1C Blastocysts carrying both gene-trap alleles (gt1/gt2) were indistinguishable from homozygous embryos for either allele (gt1/gt1 or gt2/gt2).
Specimen 1D/F: embryonic day 3.5 (more )
Note: Triple labeled: green - Nanog; red - Cdx2 (assay MGI:6272733); blue - DAPI.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 1D , 1F Authors report expressing and non-expressing cells arranged in a 'salt-and-pepper'manner.
Specimen 1G/I: embryonic day 3.5; NanogGt(D042B07)Wrst/NanogGt(D047A12)Wrst (more )
Note: Embryo had hatched and attached to uterine wall at the time of isolation, and was directly dissected from the uterus. Triple labeled: green - Nanog; red - Oct4 (Pou5f1 assay MGI:6272734); blue - DAPI.
Structure Level Pattern Image Note
TS4: blastocyst Absent 1G , 1I Blastocysts carrying both gene-trap alleles (gt1/gt2) were indistinguishable from homozygous embryos for either allele (gt1/gt1 or gt2/gt2).
Specimen 1J/L: embryonic day 3.5 (more )
Note: Embryo had hatched and attached to uterine wall at the time of isolation, and was directly dissected from the uterus. Triple labeled: green - Nanog; red - Oct4 (Pou5f1 assay MGI:6272734); blue - DAPI.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 1J , 1L Authors report expressing and non-expressing cells arranged in a 'salt-and-pepper'manner.
Specimen 2A/D: embryonic day 4.5; NanogGt(D042B07)Wrst/NanogGt(D047A12)Wrst (more )
Note: Embryo had hatched and attached to uterine wall at the time of isolation, and was directly dissected from the uterus. Triple labeled: green - Nanog; red - Oct4 (Pou5f1 assay MGI:6272734); blue - DAPI.
Structure Level Pattern Image Note
TS6: blastocyst Absent 2A , 2D
TS6: epiblast Absent 2A , 2D
Specimen 2E/H: embryonic day 4.5 (more )
Note: Embryo had hatched and attached to uterine wall at the time of isolation, and was directly dissected from the uterus. Triple labeled: green - Nanog; red - Oct4 (Pou5f1 assay MGI:6272734); blue - DAPI.
Structure Level Pattern Image Note
TS6: epiblast Present Regionally restricted 2H , 2E Expression confined to epiblast. Distinct populations of expressing and non-expressing cells.
TS6: primitive endoderm Absent 2H , 2E
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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