Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119781
Vreugdenhil E, et al., Doublecortin-like, a microtubule-associated protein expressed in radial glia, is crucial for neuronal precursor division and radial process stability. Eur J Neurosci. 2007 Feb;25(3):635-48
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6267040
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Gene symbol:
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Vim
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Gene name:
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vimentin
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Antibody:
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Anti-Vimentin
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Detection system:
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Secondary antibody coupled to Alexa Fluor 647
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Results
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Specimen
6G:
embryonic day 13.0
(more  )
Note: Double labeled: purple - Vim; red - Dclk1 (assay MGI:6267023).
Specimen
6G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Vim; red - Dclk1 (assay MGI:6267023).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon ventricular layer |
Present |
Regionally restricted |
6J
,
6G
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Expression was detected in radial glia; there was some overlap with Dclk1-immunolabel.
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Specimen
6K:
embryonic day 11.0
(more )
Note: Triple labeled: red/white - Vim; red/white - Dclk1 (assay MGI:6267023); green/white - Dcx (assay MGI:6267029).
Specimen
6K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: red/white - Vim; red/white - Dclk1 (assay MGI:6267023); green/white - Dcx (assay MGI:6267029).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
6K
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Expression was detected in radial glial cells throughout the neuroepithelium and ventricular zone, where there was overlap with Dclk1 expression. The second proliferative layer between the subventricular zone and primitive plexiform zone included cells that were immunopositive for Dclk1, Vim, and Dcx.
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| TS18: telencephalon ventricular layer |
Present |
Regionally restricted |
6K
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Expression was detected in radial glial cells throughout the neuroepithelium and ventricular zone, where there was overlap with Dclk1 expression. The second proliferative layer between the subventricular zone and primitive plexiform zone included cells that were immunopositive for Dclk1, Vim, and Dcx.
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Specimen
6N:
embryonic day 11.0
(more )
Note: Double labeled: purple - Vim; green - Dcx (assay MGI:6267029).
Specimen
6N:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Vim; green - Dcx (assay MGI:6267029).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: neural tube |
Present |
Regionally restricted |
6N
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Expression was detected in radial glia. Only very rarely was any co-labeling observed with Dcx.
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Analysis Tools