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Gene Expression Data
Assay Details
Assay
Reference: J:260421 Mesman S, et al., Expression analyzes of early factors in midbrain differentiation programs. Gene Expr Patterns. 2018 Jan;27:8-15
Assay type: RNA in situ
MGI Accession ID: MGI:6258324
Gene symbol: Mest
Gene name: mesoderm specific transcript
Probe: Mest probe3
Probe preparation: labelled with digoxigenin
Visualized with: Alkaline phosphatase
Results
Specimen 2A E11.5 Lateral: embryonic day 11.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain Present 2A E11.5 Lateral Expression in midbrain from E11.5 onwards.
TS19: midbrain-hindbrain junction Present 2A E11.5 Lateral Expression at the isthmic organizer.
TS19: prosomere 1 Present 2A E11.5 Lateral Expression continues to the prosomere 3 area.
TS19: prosomere 2 Present 2A E11.5 Lateral Expression continues to the prosomere 3 area.
TS19: prosomere 3 Present 2A E11.5 Lateral Expression continues to the prosomere 3 area.
Specimen 2A E11.5 Medial: embryonic day 11.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain Present 2A E11.5 Medial Expression in midbrain from E11.5 onwards.
TS19: midbrain-hindbrain junction Present 2A E11.5 Medial Expression at the isthmic organizer.
TS19: prosomere 1 Present 2A E11.5 Medial Expression continues to the prosomere 3 area.
TS19: prosomere 2 Present 2A E11.5 Medial Expression continues to the prosomere 3 area.
TS19: prosomere 3 Present 2A E11.5 Medial Expression continues to the prosomere 3 area.
Specimen 2A E12.5 Lateral: embryonic day 12.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 2A E12.5 Lateral Expression shifts to hindbrain in lateral parts of brain.
Specimen 2A E12.5 Medial: embryonic day 12.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 2A E12.5 Medial Expression shifts to more caudal and medial regions of midbrain. Expression borders and slightly overlaps with Th expression.
Specimen 2A1: embryonic day 12.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 2A1 Expression borders and slightly overlaps with Th expression.
Specimen 2A E14.5 Lateral: embryonic day 14.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 2A E14.5 Lateral In lateral areas, broad band of expression in intermediate stratum of midbrain, partially overlapping with most lateral parts of mdDA (mesodiencephalic dopaminergic) neurons.
Specimen 2A E14.5 Medial: embryonic day 14.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Strong Regionally restricted 2A E14.5 Medial Strong expression in ventricular zone, but reduced to a small band.
TS22: prosomere 2 Weak 2A E14.5 Medial Some expression in prosomere 2 and 3 areas.
TS22: prosomere 3 Weak 2A E14.5 Medial Some expression in prosomere 2 and 3 areas.
Specimen 2A2: embryonic day 14.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present Regionally restricted 2A2 Most overlap with Th expression occurs in ventricular zone of medial part of midbrain.
Specimen 2B E11.5 Rostral: embryonic day 11.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain mantle layer Present Regionally restricted 2B E11.5 Rostal Expression ventricular zone and subventricular/mantle zone of floor plate and part of basal plate of midbrain area.
TS19: midbrain ventricular layer Present Regionally restricted 2B E11.5 Rostal Expression ventricular zone and subventricular/mantle zone of floor plate and part of basal plate of midbrain area.
Specimen 2B E11.5 Caudal: embryonic day 11.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS19: midbrain floor plate Present Regionally restricted 2B E11.5 Caudal Expression ventricular zone and subventricular/mantle zone of floor plate and part of basal plate of midbrain area.
Specimen 2B E12.5 Rostral: embryonic day 12.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain floor plate Present Regionally restricted 2B E12.5 Rostral Expression ventricular zone and subventricular/mantle zone of floor plate and part of basal plate of midbrain area. Overlap with Th expression mainly in medial part of floor plate.
Specimen 2B E12.5 Caudal: embryonic day 12.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present Regionally restricted 2B E12.5 Caudal
TS20: midbrain ventricular layer Present Regionally restricted 2B E12.5 Caudal
Specimen 2B1: embryonic day 12.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS20: midbrain floor plate Present Regionally restricted 2B1 Overlap with Th expression mainly in medial part of floor plate.
Specimen 2B E14.5 Rostral: embryonic day 14.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain Present Regionally restricted 2B E14.5 Rostral Most overlap with Th expression occurs in ventricular zone of medial part of midbrain.
Specimen 2B E14.5 Caudal: embryonic day 14.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present Regionally restricted 2B E14.5 Caudal Most overlap with Th expression occurs in ventricular zone of medial part of midbrain.
Specimen 2B2: embryonic day 14.5 (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS22: midbrain floor plate Present Regionally restricted 2B2 Overlap with Th expression mainly in medial part of floor plate.
Specimen 2B Adult: postnatal adult (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS28: substantia nigra Weak Regionally restricted 2B Adult
Specimen 2B3: postnatal adult (more )
Note: Double labeled: blue - Mest; brown - Th (assay MGI:6258336).
Structure Level Pattern Image Note
TS28: substantia nigra Weak Regionally restricted 2B3
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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