Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:44409
Gofflot F, et al., Genetic patterning of the developing mouse tail at the time of posterior neuropore closure. Dev Dyn. 1997 Dec;210(4):431-45
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6226068
|
Gene symbol:
|
Foxa1
|
Gene name:
|
forkhead box A1
|
Probe:
|
Hnf3a probe3
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
2B:
embryonic day 10.5
(more )
Note: 28-30 somite stage.
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
28-30 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: notochord |
Present |
|
2B
|
|
TS17: hindgut epithelium |
Weak |
Patchy |
2B
|
Weak, patchy expression was observed in the caudal end of the hindgut, concentrated on the apical aspect of the epithelium.
|
TS17: cloaca |
Strong |
Regionally restricted |
2B
|
Strong signal was confined to the cranial part of the dilated cloacal region.
|
TS17: gut |
Very strong |
Regionally restricted |
2B
|
Very strong signal was continuous along the gut cranial to the cloaca.
|
TS17: future spinal cord |
Weak |
Non-Uniform |
2B
|
Weak signal is detected in the apical region of the neuroepithelium, although this is barely detectable in the more caudal neural tube.
|
TS17: neural tube |
Trace |
Regionally restricted |
2B
|
|
Specimen
3a:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Specimen
3b:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Specimen
3c:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Specimen
3d:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3d:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
Specimen
3e:
embryonic day 10.5
(more )
Note: Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
Specimen
3e:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Note:
|
Fig. 3 sections are presented in a caudal to cranial sequence (a-e).
|
|