Probe:	Chd7-3
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Similar results were obtained with two other probes: MGI:6220455 and MGI:6220456.

Structure	Level	Pattern	Image	Note
TS20: semicircular canal	Present	Regionally restricted	2B	Expression was detected in the developing canal pouches of the vestibular system.
TS20: endolymphatic sac	Absent		2B
TS20: vestibulo-cochlear ganglion	Strong		2B

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Strong		2C
TS20: cochlea	Weak		2C
TS20: inner ear mesenchyme	Present		2C	Expression was observed in the mesenchyme surrounding the vestibular system and the cochlea and the cartilage primordium of the temporal bone, but at a lower level than in the otic epithelia.
TS20: inner ear epithelium	Present		2C

Structure	Level	Pattern	Image	Note
TS22: inner ear vestibular component	Strong	Regionally restricted	2D	Strong signal was detected in the developing sensory patches in the vestibular system.
TS22: semicircular canal	Strong	Regionally restricted	2D	Strong expression was detected in the cristae.
TS22: cochlea	Present	Regionally restricted	2D	In the cochlea, expression appeared stronger than at E12.5, especially in the developing organ of Corti.
TS22: vestibulo-cochlear ganglion	Strong		2D
TS22: inner ear mesenchyme	Present		2D	Expression was observed in the mesenchyme surrounding the vestibular system and the cochlea and the cartilage primordium of the temporal bone, but at a lower level than in the otic epithelia.
TS22: inner ear epithelium	Present		2D

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present	Regionally restricted	2E	In the cochlea, expression appeared stronger than at E12.5, especially in the developing organ of Corti.
TS22: vestibulocochlear nerve cochlear component	Present		2E

Structure	Level	Pattern	Image	Note
TS24: cochlea	Strong		2F

Structure	Level	Pattern	Image	Note
TS24: semicircular canal	Present	Regionally restricted		Expression was detected in the cristae.
TS24: organ of Corti	Present
TS24: vestibulo-cochlear ganglion	Present

Structure	Level	Pattern	Image	Note
TS20: brain	Present		3A
TS20: olfactory epithelium	Strong		3A
TS20: ganglion	Present	Regionally restricted	3A	Expression was detected in olfactory ganglion.
TS20: face mesenchyme	Weak		3A
TS20: ganglionic eminence	Weak		3A
TS20: remnant of Rathke's pouch	Weak		3A
TS20: tongue	Weak		3A
TS20: neocortex	Present	Graded	3A	Graded expression was detected, with highest expression levels in proximity to the ventricles.
TS20: midbrain roof plate	Present	Graded	3A	Graded expression was detected in the tectum, with highest expression levels in proximity to the ventricles.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata ventricular layer	Present	Graded	3B	Graded expression was detected, with highest expression levels in proximity to the ventricles.
TS20: choroid plexus	Weak		3B
TS20: 4th ventricle	Weak	Regionally restricted	3B	Lower level expression was detected in the roof of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Strong		3C
TS20: ganglion	Present	Regionally restricted	3C	Expression was detected in the olfactory ganglion.

Structure	Level	Pattern	Image	Note
TS20: facial ganglion	Present
TS20: lens vesicle	Present
TS20: eye	Present	Regionally restricted		Expression was detected in the neuroectodermal layer.
TS20: cardiac muscle tissue	Absent

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		3D
TS20: lung epithelium	Strong		3D

Structure	Level	Pattern	Image	Note
TS20: intestine epithelium	Present		3E
TS20: metanephros	Present		3E
TS20: stomach epithelium	Present		3E
TS20: enteric nervous system	Present		3E	Expression was detected in the Meissner and Auerbach's plexi.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Weak		3F

Structure	Level	Pattern	Image	Note
TS10: yolk sac	Absent

Structure	Level	Pattern	Image	Note
TS13: yolk sac blood vessel	Present		3G

Structure	Level	Pattern	Image	Note
TS22: medial ganglionic eminence	Present		3H
TS22: medulla oblongata ventricular layer	Present		3H
TS22: olfactory epithelium	Strong		3H
TS22: cerebellum layer	Present	Regionally restricted	3H	Expression was detected in the external granular cell layer of the cerebellum.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Strong		3I
TS22: tooth	Present		3I
TS22: oral region	Present	Regionally restricted	3I	Expression was detected in the oral ectoderm.

Structure	Level	Pattern	Image	Note
TS22: eye	Present	Regionally restricted		Expression was detected in the neuroectodermal layer.
TS22: lens	Present

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		S3