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Gene Expression Data
Assay Details
Assay
Reference: J:222304 You L, et al., Expression atlas of the multivalent epigenetic regulator Brpf1 and its requirement for survival of mouse embryos. Epigenetics. 2014 Jun;9(6):860-72
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6208797
Gene symbol: Brpf1
Gene name: bromodomain and PHD finger containing, 1
Reporter: lacZ
Direct Detection
Assay notes: Fixative was 0.2% glutaraldehyde with a post-fix in 4% paraformaldehyde.
Results
Specimen 2A: embryonic day 8.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS13: embryo Strong Ubiquitous 2A
TS13: allantois Strong 2A
TS13: ectoplacental cone Strong 2A
TS13: future brain neural fold Strong 2A
TS13: neural tube Strong 2A
TS13: yolk sac Strong 2A

Specimen 2B: embryonic day 9.75; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS16: branchial arch Strong 2B
TS16: forelimb bud Strong 2B
TS16: future spinal cord Strong 2B
TS16: yolk sac Strong 2B
TS16: embryo Weak Ubiquitous 2B

Specimen 2C: embryonic day 11.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS19: labyrinthine zone Strong Regionally restricted 2C Expression is in the placental labyrinth.
TS28: placenta Strong Regionally restricted 2C Expression is in the placental labyrinth.
TS28: decidua Absent 2C

Specimen 2D: embryonic day 11.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS19: limb Strong 2D
TS19: midbrain Strong 2D
TS19: midbrain-hindbrain junction Strong 2D
TS19: neural tube Strong 2D
TS19: retina Strong 2D
TS19: rhombic lip Strong 2D
TS19: telencephalon Strong 2D
TS19: yolk sac Strong 2D

Specimen 2E: embryonic day 12.75; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS21: yolk sac Strong 2E
TS28: placenta Strong 2E
TS21: retina Present 2E
TS21: rhombic lip Present 2E
TS21: spinal cord Present 2E
TS21: telencephalon Present 2E
TS21: limb Weak 2E
TS21: midbrain Weak 2E
TS21: midbrain-hindbrain junction Weak 2E

Specimen 3A: embryonic day 8.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS13: ectoplacental cone Strong 3A
TS13: neural tube Present 3A
TS13: blood island Present 3D , 3A

Specimen 3B: embryonic day 8.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS13: neural tube Present 3B
TS13: yolk sac Present 3B

Specimen 3C: embryonic day 8.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS13: ectoplacental cone Strong 3C

Specimen 4A: embryonic day 9.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS15: neural tube Strong 4A , 4B
TS15: somite Strong 4A , 4B
TS15: labyrinthine zone Strong 4A
TS28: placenta Strong 4A

Specimen 4C: embryonic day 9.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS15: labyrinthine zone Strong 4C
TS28: placenta Strong 4C

Specimen 4D: embryonic day 9.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS15: yolk sac Strong Regionally restricted 4D Expression is in the blood islands.

Specimen 5A: embryonic day 12.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS20: yolk sac endoderm Strong Regionally restricted 5A Expression is in the yolk sac endoderm and is absent in the blood islands.

Specimen 5B: embryonic day 12.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS20: labyrinthine zone Strong Diffuse 5B
TS28: placenta Strong Diffuse 5B

Specimen 5C: embryonic day 12.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS20: midbrain roof plate Strong 5C
TS20: hypothalamus Strong Regionally restricted 5C Expression is at the superficial striatum.
TS20: thalamus Strong Regionally restricted 5C Expression is at the superficial striatum.
TS20: spinal cord mantle layer Strong Regionally restricted 5C , 5D Expression is in the lumbosacral zone.
TS20: heart Weak 5D , 5C
TS20: lung Weak 5D , 5C
TS20: liver Absent 5D , 5C

Specimen 5E: embryonic day 12.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS20: neural retina Very strong 5E Expression is at the ganglion cell layer.

Specimen 5F: embryonic day 12.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS20: nasal epithelium Present 5F

Specimen 5G: embryonic day 12.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS20: remnant of Rathke's pouch Present 5G

Specimen 6A: embryonic day 14.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS22: neural retina Very strong Regionally restricted 6B , 6A Expression is at the ganglion cell layer.
TS22: nasal epithelium Strong 6C , 6A
TS22: nasal mesenchyme Strong Regionally restricted 6C , 6A Expression is in the nasal bone mesenchyme.
TS22: tongue Strong 6D , 6A
TS22: lower jaw molar Strong 6E , 6A
TS22: lower jaw mesenchyme Strong Regionally restricted 6A , 6E Expression is in the mesenchyme adjacent to Meckel's cartilages.

Specimen 7A: embryonic day 17.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS25: nasal epithelium Strong 7C , 7A
TS25: neural retina Present Regionally restricted 7B , 7A Expression is at the ganglion cell layer.

Specimen 7D: embryonic day 17.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS25: brown fat Very strong 7D , 7E
TS25: neck Present 7F , 7D
TS25: bladder Trace 7D Little or no expression was found in this tissue.
TS25: heart Trace 7D Little or no expression was found in this tissue.
TS25: lung Trace 7D Little or no expression was found in this tissue.
TS25: liver Absent 7D

Specimen 7G: embryonic day 17.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS25: nose Strong Regionally restricted 7G Expression is in the back of the nose.

Specimen 7H: embryonic day 17.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS25: renal pelvis Strong 7H

Specimen 8A: postnatal day 0; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS27: nasal epithelium Strong 8A , 8C
TS27: retina Weak 8A , 8B

Specimen 8D: postnatal day 0; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS27: sphenopalatine ganglion Strong 8E , 8D
TS27: dorsal root ganglion Strong 8D

Specimen 8F: postnatal day 3; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS27: neocortex Strong 8G , 8F
TS27: hippocampus pyramidal cell layer Strong 8F , 8H
TS27: dentate gyrus granule cell layer Absent 8H , 8F
TS27: cerebellum Purkinje cell layer Strong 8H , 8I
TS27: cerebellum external granule cell layer Strong 8H , 8I

Specimen 8J: postnatal day 3; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS27: telencephalon septum Strong 8J

Specimen S2A: postnatal adult; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS28: neocortex Strong Uniform S2C , S2B , S2A
TS28: CA1 pyramidal cell layer Very strong S2A , S2C , S2B
TS28: dentate gyrus granule cell layer Very strong S2A , S2C , S2B
TS28: subiculum Strong S2B , S2A , S2C
TS28: main olfactory bulb Strong S2B
TS28: main olfactory bulb mitral cell layer Strong S2B
TS28: cerebellum Purkinje cell layer Strong S2B
TS28: cerebellum internal granule cell layer Strong S2B
TS28: accessory olfactory bulb Present S2B

Specimen not shown E9.75: embryonic day 9.75; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS16: ectoplacental cone Strong

Specimen not shown E17.5: embryonic day 17.5; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS25: trigeminal ganglion Present

Specimen not shown: postnatal day 3; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS27: main olfactory bulb mitral cell layer Strong

Specimen Table S1: postnatal day 39; Brpf1tm1a(EUCOMM)Wtsi/Brpf1+ (more )
Structure Level Pattern Image Note
TS28: brain Strong
TS28: testis Moderate
TS28: metanephros Weak
TS28: spinal cord Weak
TS28: stomach Weak
TS28: thymus Weak
TS28: bladder Absent
TS28: blood vessel Absent
TS28: heart Absent
TS28: liver Absent
TS28: lung Absent
TS28: pancreas Absent
TS28: skeletal muscle Absent
TS28: skin Absent
TS28: small intestine Absent
TS28: tongue Absent
TS28: white fat Absent


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory