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Gene Expression Data
Assay Details
Assay
Reference: J:35171 Hofmann C, et al., Analysis of limb patterning in BMP-7-deficient mice. Dev Genet. 1996;19(1):43-50
Assay type: RNA in situ
MGI Accession ID: MGI:6203071
Gene symbol: Bmp7
Gene name: bone morphogenetic protein 7
Probe: Bmp-7 cDNA4
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: forelimb bud Present Widespread 1A Expressed in the entire forelimb field.
TS15: branchial arch Present 1A
TS15: cephalic midbrain flexure Present 1A
TS15: future forebrain Present 1A
TS15: heart Present 1A
TS15: nephric duct Present 1A
TS15: otocyst Present 1A

Specimen 1B: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: forelimb bud mesenchyme Present Non-Uniform 1B Expression levels were higher in anterior than in posterior mesenchyme.
TS16: hindlimb bud Present Widespread 1B Expressed in the entire hindlimb field.

Specimen 1C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 1C
TS17: hindlimb bud apical ectodermal ridge Present 1C
TS17: forelimb bud mesenchyme Present Regionally restricted 1C Expression was found particularly in an anterior and a posterior expression domain underlying the apical ectodermal ridge, slightly more prominent anteriorly.
TS17: hindlimb bud mesenchyme Present 1C No distinction of an anterior and a posterior expression domain yet.

Specimen 1D: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: hindlimb bud apical ectodermal ridge Weak 1D
TS18: hindlimb bud mesenchyme Trace 1D Very weak expression in the mesenchyme underlying the apical ectodermal ridge.

Specimen 1E: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: forelimb bud mesenchyme Present Regionally restricted 1E Expressed in the distal mesenchyme and in regions of condensing cartilage, being more abundant in the posterior domains. An anterior expression domain was also observed at the base of the limb buds.
TS19: hindlimb bud mesenchyme Present Regionally restricted 1E Expressed in the distal mesenchyme and in regions of condensing cartilage, being more abundant in the posterior domains. An anterior expression domain was also observed at the base of the limb buds.

Specimen 1F: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Strong Regionally restricted 1F Expressed strongly at the anterior base.
TS20: hindlimb mesenchyme Strong Regionally restricted 1F Expressed strongly at the anterior base.
TS20: forelimb interdigital region mesenchyme Strong 1F
TS20: hindlimb interdigital region mesenchyme Strong 1F
TS20: forelimb pre-cartilage condensation Present 1F
TS20: hindlimb pre-cartilage condensation Present 1F


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/30/2024
MGI 6.23
The Jackson Laboratory