Tg(Cd3d-CD4,H2-Ea-HLA-DRA*0101,-HLA-DRB1*0401)#Lfug
Transgene Detail
|
|
| Symbol: |
Tg(Cd3d-CD4,H2-Ea-HLA-DRA*0101,-HLA-DRB1*0401)#Lfug |
| Name: |
transgene insertion, Lars Fugger |
| MGI ID: |
MGI:6202583 |
| Synonyms: |
DR4tg |
| Transgene: |
Tg(Cd3d-CD4,H2-Ea-HLA-DRA*0101,-HLA-DRB1*0401)#Lfug Location: unknown
|
| Alliance: |
Tg(Cd3d-CD4,H2-Ea-HLA-DRA*0101,-HLA-DRB1*0401)#Lfug page
|
|
|
|
| Transgene Type: |
|
Transgenic (Humanized sequence, Inserted expressed sequence) |
| Mutation: |
|
Insertion
|
| |
|
Tg(Cd3d-CD4,H2-Ea-HLA-DRA*0101,-HLA-DRB1*0401)#Lfug expresses
2 genes
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
CD4 (920) |
|
mouse promoter, human cDNA and gene |
| human |
HLA-DRA (3122) |
|
mouse promoter, human cDNA |
|
| |
|
Mutation details: One transgene construct consists of human HLA-DRA*0101 and HLA- DRB1*0401 (Gly-86) cDNAs, each placed downstream of the histocompatibility 2, class II antigen E alpha promoter and the rabbit beta-globin intron and upstream of the beta-globin polyadenylylation site. The other transgene construct is composed of the mouse CD3 antigen delta polypeptide promoter followed by a human CD4 cDNA fragment encoding exons 2-4 and part of 5, a genomic fragment encoding the remainder of CD4 exon 5, exons 6-9, and part of exon 10, and SV40 polyadenylation signal sequences. The two constructs were co-microinjected into single-cell embryos. Five triple-transgenic lines were established, of which one was chosen for further study. The chosen line has approximately five copies of the HLA construct and two or three copies of the CD4 construct, with both constructs in strong linkage disequilibrium. Flow cytometry experiments showed expression of the human transgenes in peripheral blood mononuclear cells. Mice with this allele express the HLA-DR4 human serotype.
(J:265804)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
|
| Original: |
J:265804 Fugger L, et al., Expression of HLA-DR4 and human CD4 transgenes in mice determines the variable region beta-chain T-cell repertoire and mediates an HLA-DR-restricted immune response. Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6151-5 |
| All: |
2 reference(s) |
|
Analysis Tools
