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Gene Expression Data
Assay Details
Assay
Reference: J:237539 He Z, et al., VEGF regulates relative allocation of Isl1+ cardiac progenitors to myocardial and endocardial lineages. Mech Dev. 2016 Nov;142:40-49
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6202322
Gene symbol: Nkx2-5
Gene name: NK2 homeobox 5
Antibody: anti-Nkx-2.5 (N-19): sc-8697
Detection system: Secondary antibody coupled to Cy2
Results
Specimen 1A left top: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present Regionally restricted 1A left top Expression is in cardiomyocyte progenitor cells.
Specimen 1A left bottom: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract cardiac muscle Present 1A left bottom
Specimen 1A right top: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present Regionally restricted 1A right top Expression is in bipotent cardiac progenitor cells.
Specimen 1A right middle: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: endocardial lining Absent 1A right middle
Specimen 1A right bottom: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: endocardial lining Absent 1A right bottom
Specimen 1B left: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract distal part Present Regionally restricted 1B left , 1B middle Expression is in cardiomyocyte progenitor cells.
Specimen 2A I: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2A I
Specimen 2A II: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2A bottom , 2A II
Specimen 2B: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B
Specimen 2B 1: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B 1
Specimen 2B 2: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B 2
Specimen 2B 3: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B 3
Specimen 2B 4: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B 4
Specimen 2B 5: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B 5
Specimen 2B 6: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B 6
Specimen 2B 7: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B 7
Specimen 2B 8: embryonic day 10.5 (more )
Note: Triple labeled: green - Nkx2-5; red - Isl1 (assay MGI:6202323); blue - Kdr (assay MGI:6202324).
Structure Level Pattern Image Note
TS17: outflow tract Present 2B 8
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory