Isl1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:237539 He Z, et al., VEGF regulates relative allocation of Isl1+ cardiac progenitors to myocardial and endocardial lineages. Mech Dev. 2016 Nov;142:40-49
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6202323
Gene symbol:	Isl1
Gene name:	ISL1 transcription factor, LIM/homeodomain

Antibody:	clone 39.4D5
Detection system:	Secondary antibody coupled to Cy3

Results

1A left top ©

Specimen 1A left top: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present	Regionally restricted	1A left top	Expression is in cardiomyocyte progenitor cells.

1A left bottom ©

Specimen 1A left bottom: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present	Regionally restricted	1A left bottom	Expression is in cardiomyocyte progenitor cells.
TS17: outflow tract cardiac muscle	Absent		1A left bottom

1A right top ©

Specimen 1A right top: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present	Regionally restricted	1A right top	Expression is in bipotent cardiac progenitor cells.

1A right middle ©

Specimen 1A right middle: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present	Regionally restricted	1A right middle	Expression is in endocardial progenitor cells.

1A right bottom ©

Specimen 1A right bottom: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present	Regionally restricted	1A right bottom	Expression is in endocardial progenitor cells.
TS17: endocardial lining	Absent		1A right bottom

1B left ©

1B middle ©

Specimen 1B left: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract distal part	Present	Regionally restricted	1B middle , 1B left	Expression is in bipotent cardiac progenitor cells.

2A I ©

Specimen 2A I: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2A I

2A bottom ©

2A II ©

Specimen 2A II: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2A II , 2A bottom

2B ©

Specimen 2B: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B

2B 1 ©

Specimen 2B 1: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B 1

2B 2 ©

Specimen 2B 2: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B 2

2B 3 ©

Specimen 2B 3: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B 3

2B 4 ©

Specimen 2B 4: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B 4

2B 5 ©

Specimen 2B 5: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B 5

2B 6 ©

Specimen 2B 6: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B 6

2B 7 ©

Specimen 2B 7: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B 7

2B 8 ©

Specimen 2B 8: embryonic day 10.5 (more

)
Note: Triple labeled: red - Isl1; green - Nkx2-5 (assay MGI:6202322); blue - Kdr (assay MGI:6202324).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		2B 8

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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