Gene Expression Data
Assay Details
Assay
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Reference:
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J:112915
Kruger M, et al., Functional redundancy of NSCL-1 and NeuroD during development of the petrosal and vestibulocochlear ganglia. Eur J Neurosci. 2006 Sep;24(6):1581-90
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6202089
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Gene symbol:
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Nhlh2
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Gene name:
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nescient helix loop helix 2
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
16-18 somites not shown:
embryonic day 9.0; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
16-18 somites not shown:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS14: trigeminal neural crest |
Present |
|
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Authors report expression first detected in trigeminal ganglion at 16-18 somite stage.
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Specimen
E9.25 (22-24 somites) not shown:
embryonic day 9.25; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
E9.25 (22-24 somites) not shown:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 9.25
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS14: facio-acoustic neural crest |
Present |
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Authors report no discernible difference between NSCL2 (Nhlh2) and NSCL1 (Nnlh1) reporter expression in any distal or proximal ganglia at E9.25 (2224 somites stage).
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TS14: epibranchial placode |
Present |
Regionally restricted |
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Authors report no discernible difference between NSCL2 (Nhlh2) and NSCL1 (Nnlh1) reporter expression in any distal or proximal ganglia at E9.25 (2224 somites stage).
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TS14: petrosal placode |
Present |
Regionally restricted |
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Authors report no discernible difference between NSCL2 (Nhlh2) and NSCL1 (Nnlh1) reporter expression in any distal or proximal ganglia at E9.25 (2224 somites stage).
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Specimen
1D:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: facio-acoustic preganglion complex |
Weak |
Regionally restricted |
1D
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Weak expression in aggregating neurons of developing vestibulocochlear ganglion.
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TS15: otocyst |
Absent |
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1D
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Expression only in cells that had left the otic vesicle.
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Specimen
1E:
embryonic day 10.5; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
1E:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: acoustic ganglion |
Strong |
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1E
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Authors state expression level increased during next few days. Strongest expression at E10.5 in geniculate (VII) and vestibulocochlear (VIII) ganglia.
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TS17: facial ganglion |
Strong |
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1E
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Authors state expression level increased during next few days. Strongest expression at E10.5 in geniculate (VII) and vestibulocochlear (VIII) ganglia.
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Specimen
1F:
embryonic day 11.5; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
1F:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: acoustic ganglion |
Present |
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1F
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From E11.5 downwards, levels of expression continuously decreased but could still be detected until the end of fetal development.
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TS19: facial ganglion |
Present |
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1F
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From E11.5 downwards, levels of expression continuously decreased but could still be detected until the end of fetal development.
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Specimen
2E:
embryonic day 18.5; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
2E:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2E inset:
embryonic day 18.5; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
2E inset:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Absent |
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2E inset
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No expression in sensory hair cells of the organ of Corti.
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Specimen
6B:
embryonic day 14.5; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
6B:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: neural retina |
Present |
Regionally restricted |
6B
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Authors report first expression detected in the ganglion cell layer at E14.5.
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Specimen
6D:
embryonic day 16.5; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
6D:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6F:
embryonic day 18.5; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
6F:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6H:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
adult not shown:
postnatal adult; Nhlh2tm1Thbr/Nhlh2 +
(more )
Specimen
adult not shown:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Nhlh2tm1Thbr/Nhlh2+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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