About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:112915 Kruger M, et al., Functional redundancy of NSCL-1 and NeuroD during development of the petrosal and vestibulocochlear ganglia. Eur J Neurosci. 2006 Sep;24(6):1581-90
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6202089
Gene symbol: Nhlh2
Gene name: nescient helix loop helix 2
Reporter: lacZ
Direct Detection
Results
Specimen 16-18 somites not shown: embryonic day 9.0; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS14: trigeminal neural crest Present Authors report expression first detected in trigeminal ganglion at 16-18 somite stage.

Specimen E9.25 (22-24 somites) not shown: embryonic day 9.25; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS14: facio-acoustic neural crest Present Authors report no discernible difference between NSCL2 (Nhlh2) and NSCL1 (Nnlh1) reporter expression in any distal or proximal ganglia at E9.25 (2224 somites stage).
TS14: epibranchial placode Present Regionally restricted Authors report no discernible difference between NSCL2 (Nhlh2) and NSCL1 (Nnlh1) reporter expression in any distal or proximal ganglia at E9.25 (2224 somites stage).
TS14: petrosal placode Present Regionally restricted Authors report no discernible difference between NSCL2 (Nhlh2) and NSCL1 (Nnlh1) reporter expression in any distal or proximal ganglia at E9.25 (2224 somites stage).

Specimen 1D: embryonic day 9.5; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS15: facio-acoustic preganglion complex Weak Regionally restricted 1D Weak expression in aggregating neurons of developing vestibulocochlear ganglion.
TS15: otocyst Absent 1D Expression only in cells that had left the otic vesicle.

Specimen 1E: embryonic day 10.5; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS17: acoustic ganglion Strong 1E Authors state expression level increased during next few days. Strongest expression at E10.5 in geniculate (VII) and vestibulocochlear (VIII) ganglia.
TS17: facial ganglion Strong 1E Authors state expression level increased during next few days. Strongest expression at E10.5 in geniculate (VII) and vestibulocochlear (VIII) ganglia.

Specimen 1F: embryonic day 11.5; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS19: acoustic ganglion Present 1F From E11.5 downwards, levels of expression continuously decreased but could still be detected until the end of fetal development.
TS19: facial ganglion Present 1F From E11.5 downwards, levels of expression continuously decreased but could still be detected until the end of fetal development.

Specimen 2E: embryonic day 18.5; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS26: vestibulocochlear nerve Weak Regionally restricted 2E

Specimen 2E inset: embryonic day 18.5; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS26: organ of Corti Absent 2E inset No expression in sensory hair cells of the organ of Corti.

Specimen 6B: embryonic day 14.5; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 6B Authors report first expression detected in the ganglion cell layer at E14.5.

Specimen 6D: embryonic day 16.5; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS24: retina layer Present Regionally restricted 6D Authors report expression in the ganglion cell layer.
TS24: retina inner nuclear layer Strong 6D Strongest expression at inner boundary of ventricular zone.

Specimen 6F: embryonic day 18.5; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS26: retina outer nuclear layer Ambiguous 6F Authors report virtually no expression in outer ventricular zone.

Specimen 6H: postnatal day 10; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS28: retina outer nuclear layer Absent 6H No expression in outer ventricular zone.
TS28: retina ganglion cell layer Present 6H Expression restricted to ganglion cell layer and inner nuclear layer of retina.
TS28: retina inner nuclear layer Present 6H Expression restricted to ganglion cell layer and inner nuclear layer of retina.

Specimen adult not shown: postnatal adult; Nhlh2tm1Thbr/Nhlh2+ (more )
Structure Level Pattern Image Note
TS28: retina Absent


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/25/2025
MGI 6.24
The Jackson Laboratory