Antibody:	Anti-Matrilin-4
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: Meckel's cartilage	Present		7C
TS22: basisphenoid cartilage condensation	Present		7C
TS22: hip	Present		7C
TS22: sternum	Present		7C
TS22: vertebral cartilage condensation	Present		7C
TS22: nose	Present	Regionally restricted	7C	Expression was detected in the anlage of the nasal cavity bone.
TS22: respiratory system cartilage	Present	Regionally restricted	7C	Expression was detected in the thyroid and tracheal cartilages.
TS22: bronchiole	Present	Regionally restricted	7C	Expression was detected around the terminal bronchioli of the lung.

Structure	Level	Pattern	Image	Note
TS27: articular cartilage	Present	Regionally restricted	8A	High expression was detected in the knee.
TS27: ligament	Present	Regionally restricted	8A	High expression was detected in the knee.
TS27: perichondrium	Present	Regionally restricted	8A	High expression was detected in the knee.
TS27: periosteum	Present	Regionally restricted	8A	High expression was detected in the knee.
TS27: long bone epiphyseal plate hypertrophic zone	Present	Regionally restricted	8A	Expression was detected in the epiphyseal cartilage of the distal femur and proximal tibia in the zone of resting, proliferating, and hypertrophic cartilage.
TS27: long bone epiphyseal plate proliferative zone	Present	Regionally restricted	8A	Expression was detected in the epiphyseal cartilage of the distal femur and proximal tibia in the zone of resting, proliferating, and hypertrophic cartilage.
TS27: long bone epiphyseal plate resting zone	Present	Regionally restricted	8A	Expression was detected in the epiphyseal cartilage of the distal femur and proximal tibia in the zone of resting, proliferating, and hypertrophic cartilage.

Structure	Level	Pattern	Image	Note
TS28: femur	Strong	Regionally restricted	8B	Expression was strong in the calcified area of newly synthesized bone.
TS28: tibia	Strong	Regionally restricted	8B	Expression was strong in the calcified area of newly synthesized bone.
TS28: long bone epiphyseal plate	Strong	Regionally restricted	8B	A strong signal was noted in the epiphyseal growth plate of the tibia and femur.
TS28: long bone metaphysis	Present	Regionally restricted	8B	Expression was detected in the cartilage remnants in the metaphysis.
TS28: articular cartilage	Absent		8B

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		8C
TS27: nasal bone	Present		8C
TS27: nasal septum	Present		8C
TS27: occipital bone	Present		8C
TS27: pons	Present		8C
TS27: upper jaw incisor	Present		8C
TS27: hair follicle	Present	Regionally restricted	8C	Expression was detected in the inner and outer mesenchymal layer of hair follicles.

Structure	Level	Pattern	Image	Note
TS27: cardiac fibrous skeleton	Present		8D
TS27: clavicle	Present		8D
TS27: costal cartilage	Present		8D
TS27: manubrium sterni	Present		8D
TS27: spinal cord	Present		8D
TS27: trachea	Present		8D
TS27: xiphisternum	Present		8D
TS27: vertebra transverse process	Present		8D
TS27: vertebral body	Present		8D

Structure	Level	Pattern	Image	Note
TS27: alveolar septum	Present		8G	Expression was detected in the interalveolar walls.

Structure	Level	Pattern	Image	Note
TS27: cornea	Present		8H
TS27: corneo-scleral junction	Present		8H
TS27: sclera	Present		8H

Structure	Level	Pattern	Image	Note
TS27: heart valve	Present		8I

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		8J
TS28: corpus striatum	Present		8J
TS28: hippocampus	Present		8J
TS28: medulla oblongata	Present		8J
TS28: neocortex	Present		8J
TS28: pallidum	Present		8J
TS28: pons	Present		8J
TS28: thalamus	Present		8J

Structure	Level	Pattern	Image	Note
TS28: basement membrane of epithelium	Present	Regionally restricted	9B'	Expression was detected with the basement membrane underlying the esophageal epithelium.
TS28: esophagus	Present	Regionally restricted	9B'	Expression was detected with the basement membrane underlying the esophageal epithelium.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		9C'
TS28: cerebellum white matter	Strong		9C'