Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45849
Antczak M, et al., Oocyte influences on early development: the regulatory proteins leptin and STAT3 are polarized in mouse and human oocytes and differentially distributed within the cells of the preimplantation stage embryo. Mol Hum Reprod. 1997 Dec;3(12):1067-86
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6201106
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Gene symbol:
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Lepr
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Gene name:
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leptin receptor
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Antibody:
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OB-R antibody
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Stat3 (C-20-G) antibody MGI:6201084 may have been used in this assay.
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Results
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Specimen
6A:
postnatal adult
(more  )
Note: Metaphase II oocyte.
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Metaphase II oocyte.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Regionally restricted |
6A
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in MII oocytes, expression within cytoplasm, clustered near center of cell. Virtually no expression associatd with plasma membrane.
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Specimen
6B:
embryonic day 0.5
(more )
Note: Pronuclear egg.
Specimen
6B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Pronuclear egg.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
Regionally restricted |
6B
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Punctate expression in both cytoplasm and concentrated within the pronuclei. No nucleolar expression.
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Specimen
6C:
embryonic day 1.0
(more )
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
6C
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Expression in primarily nucleus-associated, with diffuse cytoplasmic expression in each blastomere. Virtually no expression associatd with plasma membrane. No nucleolar expression.
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