Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:193806
Garcia-Tunon I, et al., Association of Rex-1 to target genes supports its interaction with Polycomb function. Stem Cell Res. 2011 Jul;7(1):1-16
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6199710
|
|
Gene symbol:
|
Zfp42
|
|
Gene name:
|
zinc finger protein 42
|
|
Antibody:
|
anti-Rex1/Zfp42
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to rhodamine
|
|
|
Results
|
Specimen
1C/D:
embryonic day 3.5
(more  )
Note: Double labeled: red - Zfp42; green - Cdx2 (assay MGI:6199709).
Specimen
1C/D:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 3.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Paraformaldehyde
|
|
Note:
|
Double labeled: red - Zfp42; green - Cdx2 (assay MGI:6199709).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
Widespread |
1C
,
1D
|
Expressed in all cells in a pattern that comprises a combination of cytoplasmic, perinuclear and nuclear localization. Nuclear staining was apparent, but weaker as compared to stronger staining in the cytoplasm.
|
| TS4: inner cell mass |
Present |
Widespread |
1D
,
1C
|
|
| TS4: trophectoderm |
Present |
Widespread |
1D
,
1C
|
|
Specimen
1G/H:
embryonic day 3.5
(more )
Note: Double labeled: red - Zfp42; green - Cdx2 (assay MGI:6199709).
Specimen
1G/H:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 3.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Paraformaldehyde
|
|
Note:
|
Double labeled: red - Zfp42; green - Cdx2 (assay MGI:6199709).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
Widespread |
1H
,
1G
|
Expressed in all cells in a pattern that comprises a combination of cytoplasmic, perinuclear and nuclear localization. Nuclear staining was apparent, but weaker as compared to stronger staining in the cytoplasm.
|
| TS4: inner cell mass |
Present |
Widespread |
1G
,
1H
|
|
| TS4: trophectoderm |
Present |
Widespread |
1G
,
1H
|
|
|
Analysis Tools