Gene Expression Data
Assay Details
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Assay
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Reference:
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J:145977
Tetreault N, et al., The LIM homeobox transcription factor Lhx2 is required to specify the retina field and synergistically cooperates with Pax6 for Six6 trans-activation. Dev Biol. 2009 Mar 15;327(2):541-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6198686
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Probe:
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Pax6 probe55
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1G Pax6:
embryonic day 8.25
(more  )
Note: 2-somite stage
Specimen
1G Pax6:
(close  )
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Genetic Background:
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either: 129 or CD-1
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: eye neural ectoderm |
Present |
Regionally restricted |
1G Pax6
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Diffuse expression throughout presumptive eye domain. No expression in region of anterior retina corresponding to presumptive retina field.
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Specimen
2D -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Lhx2tm1Dra/Lhx2tm1Dra
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: eye neural ectoderm |
Absent |
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2D Lhx2-/-
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Expression absent or highly reduced in anterior neural plate in region corresponding to presumptive retina field.
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Specimen
2D wt:
embryonic day 8.25
(more )
Specimen
2D wt:
(close )
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Genetic Background:
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either: 129 or CD-1
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: eye neural ectoderm |
Present |
Regionally restricted |
2D wt
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Expression present in anterior neural plate in region corresponding to presumptive retina field.
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Analysis Tools