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Gene Expression Data
Assay Details
Assay
Reference: J:145977 Tetreault N, et al., The LIM homeobox transcription factor Lhx2 is required to specify the retina field and synergistically cooperates with Pax6 for Six6 trans-activation. Dev Biol. 2009 Mar 15;327(2):541-50
Assay type: RNA in situ
MGI Accession ID: MGI:6198685
Gene symbol: Six3
Gene name: sine oculis-related homeobox 3
Probe: Six3 probe23
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1G Six3: embryonic day 8.25 (more )
Note: 2-somite stage
Structure Level Pattern Image Note
TS13: future prosencephalon Strong Regionally restricted 1G Six3 Expression predominant in prospective pituitary/hypothalamic axis and ventral forebrain.
TS13: eye neural ectoderm Absent 1G Six3 Expression nearly absent in region of anterior retina corresponding to presumptive retina field.
Specimen 2A -/-: embryonic day 8.25; Lhx2tm1Dra/Lhx2tm1Dra (more )
Note: neural plate stage
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 2A Lhx2-/- Expression in pituitary/hypothalamic axis and ventral forebrain.
TS13: eye neural ectoderm Absent 2A Lhx2-/- No expression in retina field at neural plate stage.
Specimen 2A wt: embryonic day 8.25 (more )
Note: neural plate stage
Structure Level Pattern Image Note
TS13: future prosencephalon Strong Regionally restricted 2A wt Robust expression in presumptive pituitary/hypothalamic axis and ventral forebrain region.
TS13: eye neural ectoderm Trace 2A wt Authors state expression in retina field was only initiated.
Specimen 2B -/-: embryonic day 8.5; Lhx2tm1Dra/Lhx2tm1Dra (more )
Note: neural fold stage
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 2B Lhx2-/- Expression in presumtive pituitary/hypothalamic axis and ventral forebrain.
TS13: eye neural ectoderm Absent 2B Lhx2-/- No expression in retina field and optic sulcus at neural fold stage.
Specimen 2B wt: embryonic day 8.5 (more )
Note: neural fold stage
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 2B wt Expression in pituitary/hypothalamic axis and ventral forebrain.
TS13: eye neural ectoderm Present Regionally restricted 2B wt Authors report well-established expression in optic sulcus.
Specimen 3B -/-: embryonic day 9.0; Lhx2tm1Dra/Lhx2tm1Dra (more )
Structure Level Pattern Image Note
TS14: optic vesicle neural ectoderm Weak 3B Lhx2-/- Expression present but reduced in optic vesicle.
TS14: future telencephalon Weak 3B Lhx2-/- Expression also reduced in telencephalic vesicle.
Specimen 3B wt: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: optic vesicle neural ectoderm Present 3B wt
TS14: future telencephalon Present 3B wt Expression present in telencephalic vesicle.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory