Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Rubcnem1Dgre
Name:	RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; endonuclease-mediated mutation 1, Douglas R Green
MGI ID:	MGI:6196512
Synonyms:	Rubicon-
Gene:	Rubcn  Location: Chr16:32642072-32698121 bp, - strand  Genetic Position: Chr16, 23.38 cM
Alliance:	Rubcnem1Dgre page

Strain of Origin:

(C57BL/6N x C57BL/6J)F1

Allele Type:    Endonuclease-mediated (Null/knockout) Mutation:    Nucleotide substitutions   Mutation details: Aspartic acid codon 178 in exon 5 was changed to premature stop codon TAA using an sgRNA and a 183 nt single-stranded DNA oligonucleotide template with CRISPR/Cas9 technology. Two additional substitutions were introduced immediately downstream to create an EcoRI restriction site for diagnostic purposes. The mutation is encoded NM_001200038.1:c.562_569delinsTAAGAAT. All necessary components were microinjected into the cytoplasm of C57BL/6 zygotes. (J:261225)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

4 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rubcn Mutation:	39 strains or lines available

Original:	J:261225 Martinez J, et al., Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins. Nat Cell Biol. 2015 Jul;17(7):893-906
All:	12 reference(s)