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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Rubcnem1Dgre
endonuclease-mediated mutation 1, Douglas R Green
MGI:6196512
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Rubcnem1Dgre/Rubcnem1Dgre C57BL/6-Rubcnem1Dgre MGI:6196876
cx2
 		Rubcnem1Dgre/Rubcnem1Dgre
Tg(CAG-EGFP/Map1lc3b)53Nmz/0 		involves: C57BL/6 * C57BL/6J * C57BL/6N * C57BL/6NCrlj * DBA/2 MGI:6196878


Genotype
MGI:6196876
hm1
Allelic
Composition		 Rubcnem1Dgre/Rubcnem1Dgre
Genetic
Background		 C57BL/6-Rubcnem1Dgre
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rubcnem1Dgre mutation (1 available); any Rubcn mutation (37 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
hematopoietic system phenotype ( J:261225 )
N
• normal numbers of B, T and NK cells in spleens and lymph nodes and normal T cell subsets in thymi
abnormal macrophage physiology ( J:261225 )
• reduced recruitment of Becn1, Uvrag, Pick3c3 (VPS34), Map1lc3 (LC3-II) and Atg7 to LAP (LC3-associated phagocytosis)-engaged phagosomes
• no recruitment of Atg5-12 and Atg16l to LAP (LC3-associated phagocytosis)-engaged phagosomes
• lack of phosphatidylinositol 3-phosphate generation by Pick3c3 (VPS34) associated with LAP (LC3-associated phagocytosis)-engaged phagosomes
• failure to produce ROS (reactive oxygen species) upon zymosan stimulation
• lack of phosphorylated Ncf4 (p-p40PHOX) in LAP (LC3-associated phagocytosis)-engaged phagosomes
• levels of Cyba (p22PHOX) in LAP (LC3-associated phagocytosis)-engaged phagosomes

immune system
immune system phenotype ( J:261225 )
N
• normal numbers of B, T and NK cells in spleens and lymph nodes and normal T cell subsets in thymi
abnormal macrophage physiology ( J:261225 )
• reduced recruitment of Becn1, Uvrag, Pick3c3 (VPS34), Map1lc3 (LC3-II) and Atg7 to LAP (LC3-associated phagocytosis)-engaged phagosomes
• no recruitment of Atg5-12 and Atg16l to LAP (LC3-associated phagocytosis)-engaged phagosomes
• lack of phosphatidylinositol 3-phosphate generation by Pick3c3 (VPS34) associated with LAP (LC3-associated phagocytosis)-engaged phagosomes
• failure to produce ROS (reactive oxygen species) upon zymosan stimulation
• lack of phosphorylated Ncf4 (p-p40PHOX) in LAP (LC3-associated phagocytosis)-engaged phagosomes
• levels of Cyba (p22PHOX) in LAP (LC3-associated phagocytosis)-engaged phagosomes

mortality/aging
mortality/aging ( J:261225 )
N
• mice are born at expected Mendelian ratios




Genotype
MGI:6196878
cx2
Allelic
Composition		 Rubcnem1Dgre/Rubcnem1Dgre
Tg(CAG-EGFP/Map1lc3b)53Nmz/0
Genetic
Background		 involves: C57BL/6 * C57BL/6J * C57BL/6N * C57BL/6NCrlj * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rubcnem1Dgre mutation (1 available); any Rubcn mutation (37 available)
Tg(CAG-EGFP/Map1lc3b)53Nmz mutation (5 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal macrophage morphology ( J:261225 )
• increased numbers of LC3 (Map1lc3) puncta
abnormal macrophage physiology ( J:261225 )
• unable to translocate LC3 (Map1lc3) to LAP (LC3-associated phagocytosis)-engaged phagosome
• phagocytosis

immune system
abnormal macrophage morphology ( J:261225 )
• increased numbers of LC3 (Map1lc3) puncta
abnormal macrophage physiology ( J:261225 )
• unable to translocate LC3 (Map1lc3) to LAP (LC3-associated phagocytosis)-engaged phagosome
• phagocytosis




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
08/05/2025
MGI 6.24 		The Jackson Laboratory