Gene Expression Data
Assay Details
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Assay
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Reference:
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J:105155
Semba K, et al., A Novel Murine Gene, Sickle tail, Linked to the Danforth's short tail Locus, Is Required for Normal Development of the Intervertebral Disc. Genetics. 2006 Jan;172(1):445-56
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6195736
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Gene symbol:
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Etl4
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Gene name:
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enhancer trap locus 4
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fix - 1% formaldehyde, 0.2% glutaraldehyde.
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Results
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Specimen
4A:
(close  )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4C:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4E:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
4F:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
4K:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: nucleus pulposus |
Present |
Regionally restricted |
4L
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4K
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X-gal-positive cells were positioned in the center of the vertebrae.
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Specimen
4M:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4Gt(pU8)80211Imeg
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: nucleus pulposus |
Present |
Regionally restricted |
4M
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4N
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X-gal-positive cells in the nucleus pulposus were shifted to the periphery in the mutant.
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Specimen
4O:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
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Age:
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postnatal week 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: nucleus pulposus |
Present |
Regionally restricted |
4O
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4P
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X-gal-positive cells were positioned in the center of the vertebrae.
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Specimen
4Q:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4Gt(pU8)80211Imeg
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Age:
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postnatal week 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: nucleus pulposus |
Present |
Regionally restricted |
4R
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4Q
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X-gal-positive cells were dislocated to the left or right side in the mutant.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4Gt(pU8)80211Imeg
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7Ca:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * Danforth's duplication stock * STOCK Mcoln3Va
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
Etn2Sd/Etn2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Alleles were trans-heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
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7Ca
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The expression pattern in the tail notochord was the same in both trans and cis compound heterozygous embryos.
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Specimen
7Cb:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * Danforth's duplication stock * STOCK Mcoln3Va
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
Etn2Sd/Etn2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Alleles were trans-heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: notochord |
Present |
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7Cb
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The expression pattern in the tail notochord was the same in both trans and cis compound heterozygous embryos.
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Specimen
7Cc:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * Danforth's duplication stock * STOCK Mcoln3Va
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
Etn2Sd/Etn2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Alleles were trans-heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: notochord |
Present |
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7Cc
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The expression pattern in the tail notochord was the same in both trans and cis compound heterozygous embryos.
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Specimen
7Cd:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * Danforth's duplication stock * STOCK Mcoln3Va
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
Etn2Sd/Etn2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Alleles were cis-heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
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7Cd
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The expression pattern in the tail notochord was the same in both trans and cis compound heterozygous embryos.
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Specimen
7Ce:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * Danforth's duplication stock * STOCK Mcoln3Va
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
Etn2Sd/Etn2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Alleles were cis-heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: notochord |
Present |
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7Ce
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The expression pattern in the tail notochord was the same in both trans and cis compound heterozygous embryos.
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Specimen
7Cf:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA * Danforth's duplication stock * STOCK Mcoln3Va
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Mutant Allele(s):
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Etl4Gt(pU8)80211Imeg/Etl4+
Etn2Sd/Etn2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Alleles were cis-heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: notochord |
Present |
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7Cf
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The expression pattern in the tail notochord was the same in both trans and cis compound heterozygous embryos.
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