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Gene Expression Data
Assay Details
Assay
Reference: J:130836 Gerbe F, et al., Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst. Dev Biol. 2008 Jan 15;313(2):594-602
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6189096
Gene symbol: Nanog
Gene name: Nanog homeobox
Antibody: anti-Nanog
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-Nanog MGI:6189070 may have been used in this assay.
Results
Specimen 4A: embryonic day 3.5 (more )
Note: 24 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 4A Nanog , 4A E-Cadh , 4A Hoechst Authors report exclusive expression in inner cell mass cells at every time point analyzed, and that the propotion of expressing cells ermains nearly the same.
Specimen 4B: embryonic day 3.75 (more )
Note: 26 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 4B Hoechst , 4B Nanog , 4B E-Cadh Authors report exclusive expression in inner cell mass cells at every time point analyzed, and that the propotion of expressing cells ermains nearly the same.
Specimen 4C: embryonic day 3.75 (more )
Note: 27 cells. Triple labeled: green - Nanog; red - Lrp2 (assay MGI:6189094); white either Cdh1 (assay MGI:6189097) or Hoechst.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 4C Nanog , 4C E-Cadh , 4C Hoechst Authors report exclusive expression in inner cell mass cells at every time point analyzed, and that the propotion of expressing cells ermains nearly the same.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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