Antibody:	anti-Robo2
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Rabbit anti-Robo1 MGI:6188841 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Widespread	1_Robo2 E13.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present		1_Robo2 E13.5 Middle	Expression restricted more laterally to the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Widespread	1_Robo2 E13.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS23: basal ganglia	Present	Widespread	1_Robo2 E15.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex	Present	Widespread	1_Robo2 E15.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS23: basal ganglia	Present	Widespread	1_Robo2 E15.5 Middle	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex	Present	Widespread	1_Robo2 E15.5 Middle	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS23: basal ganglia	Present	Widespread	1_Robo2 E15.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex	Present	Widespread	1_Robo2 E15.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong	Regionally restricted		Strong expression in preplate layer.
TS23: cerebral cortex mantle layer	Strong
TS23: cortical subplate	Strong
TS23: cerebral cortex intermediate zone	Strong	Regionally restricted		Strong expression in tangential migratory routes of interneurons, especially within the intermediate zone/subventricular zone.
TS23: cerebral cortex subventricular zone	Strong	Regionally restricted		Strong expression in tangential migratory routes of interneurons, especially within the intermediate zone/subventricular zone.

Structure	Level	Pattern	Image	Note
TS25: basal ganglia	Present	Widespread	1_Robo2 E17.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex	Present	Widespread	1_Robo2 E17.5 Rostral	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS25: basal ganglia	Present	Widespread	1_Robo2 E17.5 Middle	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex	Present	Widespread	1_Robo2 E17.5 Middle	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS25: basal ganglia	Present	Widespread	1_Robo2 E17.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex	Present	Widespread	1_Robo2 E17.5 Caudal	Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted		Expression somewhat downregulated in ventral telencephalon.
TS25: cerebral cortex subventricular zone	Strong

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	2D	Expression confined to upper part of cortex.
TS23: cerebral cortex marginal layer	Strong		2D	Expression particularly strong in subplate and marginal zone.
TS23: cortical subplate	Strong		2D	Expression particularly strong in subplate and marginal zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	2E	Expression in neurons throughout the developing cortex.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	2F	Expression in neurons throughout the developing cortex.