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Gene Expression Data
Assay Details
Assay
Reference: J:169020 Zhang Z, et al., Widely expressed Af17 is likely not required for embryogenesis, hematopoiesis, and animal survival. Genesis. 2010 Dec;48(12):693-706
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6161206
Gene symbol: Mllt6
Gene name: myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6
Reporter: lacZ
Direct Detection
Results
Specimen 3a: embryonic day 10.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS17: midbrain-hindbrain junction Present 3a
TS17: olfactory placode Present Regionally restricted 3a Expressed in the margin of the olfactory pit.
TS17: forebrain Present Regionally restricted 3a Expressed in the rostral end of the forebrain around the dorsal midline.
TS17: forelimb bud Present Regionally restricted 3a Relatively weak staining at the edge of the forelimb bud.

Specimen 3b: embryonic day 10.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS17: midbrain-hindbrain junction Present 3b
TS17: olfactory placode Present Regionally restricted 3b Expressed in the margin of the olfactory pit.
TS17: forebrain Present Regionally restricted 3b Expressed in the rostral end of the forebrain around the dorsal midline.
TS17: forelimb bud Present Regionally restricted 3b Relatively weak staining at the edge.
TS17: tail Present 3b Relatively weak staining at the tail bud.

Specimen 3d: embryonic day 10.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS17: neural tube Present Regionally restricted 3d Expressed in the neuroepithelium, but not adjacent mesoderm or ectoderm.

Specimen 4a: embryonic day 12.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS20: midbrain-hindbrain junction Present 4a
TS20: central nervous system Present Regionally restricted 4a Expressed in the midline.
TS20: dorsal root ganglion Present 4a

Specimen 4b: embryonic day 12.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS20: midbrain roof plate Present Regionally restricted 4b Expressed along the middle.
TS20: central nervous system Present Regionally restricted 4b Expressed in the midline.
TS20: dorsal root ganglion Present 4b

Specimen 4c: embryonic day 12.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted 4c Expressed in the median walls of telencephalic vesicles.
TS20: nasal epithelium Present 4c

Specimen 4d: embryonic day 12.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS20: eye Present 4d

Specimen 4f: embryonic day 12.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted 4f Expressed in the median wall of the telencephalic vesicles.

Specimen 4g: embryonic day 12.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS20: eye Present 4g With the basal (lens) side of the neural retina being stained to a greater extent.

Specimen 4h: embryonic day 12.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS20: heart Strong 4h
TS20: metanephros Strong 4h
TS20: mesonephric tubule Present 4h

Specimen 4i: embryonic day 14.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS22: dorsal root ganglion Present 4i

Specimen 4k/l: embryonic day 14.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS22: dorsal root ganglion Present 4k , 4l

Specimen 5 Brain +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: brain Present 5Brain +/- Weaker expression compared to homozygous mutant.

Specimen 5 Brain -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: brain Present 5Brain -/-

Specimen 5 Kidney +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Note: Wild-type had some noticeable background X-gal staining.
Structure Level Pattern Image Note
TS24: metanephros Present 5Kidney +/- Weaker expression compared to homozygous mutant.

Specimen 5 Kidney -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Note: Wild-type had some noticeable background X-gal staining.
Structure Level Pattern Image Note
TS24: metanephros Present 5Kidney -/-

Specimen 5 Testis +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: testis Present 5Testis +/- Weaker expression compared to homozygous mutant.

Specimen 5 Testis -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: testis Present 5Testis -/-

Specimen 5 Eye +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: eye Present 5Eye +/- Weaker expression compared to homozygous mutant.

Specimen 5 Eye -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: eye Present 5Eye -/-

Specimen 5 Bladder +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: bladder Present 5Bladder +/- Weaker expression compared to homozygous mutant.

Specimen 5 Bladder -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: bladder Present 5Bladder -/- Weaker expression.

Specimen 5 Heart +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: heart Present 5Heart +/- Weaker expression compared to homozygous mutant.

Specimen 5 Heart -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: heart Present 5Heart -/-

Specimen 5 Lung +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: lung Present 5Lung +/- Weaker expression compared to homozygous mutant.

Specimen 5 Lung -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: lung Present 5Lung -/-

Specimen 5 Spleen +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: liver Absent 5Spleen +/-

Specimen 5 Spleen -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: spleen Absent 5Spleen -/-

Specimen 5 Liver +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: liver Absent 5Liver +/-

Specimen 5 Liver -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: liver Absent 5Liver -/-

Specimen 5 Stomach +/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS24: stomach Present 5Stomach +/- Weaker expression compared to homozygous mutant.

Specimen 5 Stomach -/-: embryonic day 16.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS24: stomach Present 5Stomach -/- Weaker expression.

Specimen 6a Brain: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: brain Present 6a Brain -/-

Specimen 6a Kidney: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: metanephros Present 6a Kidney -/-

Specimen 6a Testis: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: testis Present 6a Testis -/-

Specimen 6a Eye: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: eye Present 6a Eye -/-

Specimen 6a Bladder: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: bladder Present 6a Bladder -/-

Specimen 6a Heart: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: heart Present 6a Heart -/-

Specimen 6a Lung: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: lung Present 6a Lung -/-

Specimen 6a Spleen: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: spleen Present 6a Spleen -/-

Specimen 6a Penis: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: penis Absent 6a Penis -/-

Specimen 6a Liver: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: liver Absent 6a Liver -/-

Specimen 6a Small intestine: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: small intestine Absent 6a Small intestine -/-

Specimen 6a Stomach: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: stomach Present 6a Stomach -/-

Specimen 6a Salivary Gland: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: salivary gland Present 6a Salivary Gland -/-

Specimen 6a Uterus: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: uterus Present 6a Uterus -/-

Specimen 7a/b/b': postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: cerebral cortex Present 7b' , 7b , 7a
TS28: hippocampus Present 7b' , 7b , 7a
TS28: deep cerebellar nucleus Present Regionally restricted 7a , 7b' , 7b Expressed in some deep nuclei.
TS28: hippocampus region Strong 7b
TS28: dentate gyrus Ambiguous 7b' , 7a Almost complete absence of staining.

Specimen 7c/d: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: cerebellum molecular layer Present 7c , 7d
TS28: cerebellum granule cell layer Absent 7c , 7d

Specimen 7e/f: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Note: Double labeled: blue - Mllt6; brown - Gfap (assay MGI:6161210).
Structure Level Pattern Image Note
TS28: brain Present 7e , 7f Not colabeled by anti-GFAP antibody.

Specimen 7g/h: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Note: Double labeled: blue - Mllt6; brown - Rbfox3 (assay MGI:6161211).
Structure Level Pattern Image Note
TS28: hippocampus pyramidal cell layer Present 7g , 7h Largely, but not completely overlap with the staining of Rbfox3.

Specimen 7i/j: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Note: Double labeled: blue - Mllt6; brown - Rbfox3 (assay MGI:6161211).
Structure Level Pattern Image Note
TS28: cerebral cortex Present 7j , 7i Largely, but not completely overlap with the staining of Rbfox3.

Specimen 8a/b/c: embryonic day 17.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS25: dorsal root ganglion Strong 8b , 8a , 8c
TS25: spinal cord Present 8b , 8a , 8c

Specimen 8f: embryonic day 17.5; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS25: renal cortex Present Scattered 8f Sparsely expressed.
TS25: renal medulla Present Scattered 8f Sparsely expressed.

Specimen not shown: postnatal week 6-8; Mllt6Gt(AC0433)Wtsi/Mllt6Gt(AC0433)Wtsi (more )
Structure Level Pattern Image Note
TS28: bone marrow Absent

Specimen not shown: embryonic day 10.5; Mllt6Gt(AC0433)Wtsi/Mllt6+ (more )
Structure Level Pattern Image Note
TS17: forebrain Present Same pattern as in homozygous, just weaker.
TS17: forelimb bud Present Same pattern as in homozygous, just weaker.
TS17: midbrain-hindbrain junction Present Same pattern as in homozygous, just weaker.
TS17: olfactory placode Present Same pattern as in homozygous, just weaker.
TS17: tail Present Same pattern as in homozygous, just weaker.


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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory