Antibody:

Anti-Sox10

Structure	Level	Pattern	Image	Note
TS20: large intestine	Present	Regionally restricted	2E	Migrating neural crest cells were detected in the large intestine/colon.
TS20: migrating neural crest	Present	Regionally restricted	2E	Migrating neural crest cells were detected in the large intestine/colon.

Structure	Level	Pattern	Image	Note
TS20: large intestine	Present	Regionally restricted	2F	Migrating neural crest cells were detected in the large intestine/colon, but the migratory wavefront was delayed in the mutant colon compared to wild type controls.
TS20: migrating neural crest	Present	Regionally restricted	2F	Migrating neural crest cells were detected in the large intestine/colon, but the migratory wavefront was delayed in the mutant colon compared to wild type controls.

Structure	Level	Pattern	Image	Note
TS20: large intestine	Present	Regionally restricted		Migrating neural crest cells were detected in the large intestine/colon, but the migratory wavefront was delayed in the mutant colon compared to wild type controls.
TS20: migrating neural crest	Present	Regionally restricted		Migrating neural crest cells were detected in the large intestine/colon, but the migratory wavefront was delayed in the mutant colon compared to wild type controls.

Structure	Level	Pattern	Image	Note
TS17: gut	Present	Regionally restricted		Expression was detected in vagal neural crest-derived cells in the mutant gut with no delay in entry compared to wild type.
TS17: migrating neural crest	Present	Regionally restricted		Expression was detected in vagal neural crest-derived cells in the mutant gut with no delay in entry compared to wild type.

Structure	Level	Pattern	Image	Note
TS20: large intestine	Present	Regionally restricted	2O	Expression was detected in the migratory wavefront of enteric neural crest-derived cells in the mid-colon. In this sample, very few Tubb3 immunopositive neurites were associated with the most caudal Sox10+ cells.
TS20: migrating neural crest	Present	Regionally restricted	2O	Expression was detected in the migratory wavefront of enteric neural crest-derived cells in the mid-colon. In this sample, very few Tubb3 immunopositive neurites were associated with the most caudal Sox10+ cells.

Structure	Level	Pattern	Image	Note
TS20: large intestine	Present	Regionally restricted	2P	Expression was detected in the migratory wavefront of enteric neural crest-derived cells in the mid-colon. There was variability in the number of Tuj1+ neurites at the wavefront; this sample showed Tuj1+ neurites associated with most of the Sox10+ cells.
TS20: migrating neural crest	Present	Regionally restricted	2P	Expression was detected in the migratory wavefront of enteric neural crest-derived cells in the mid-colon. There was variability in the number of Tuj1+ neurites at the wavefront; this sample showed Tuj1+ neurites associated with most of the Sox10+ cells.

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	4A'	Expression was detected in all non-neuronal enteric neural crest-derived cells.

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	4B'	Expression was detected in all non-neuronal enteric neural crest-derived cells.

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	4E'	Expression was detected in all non-neuronal enteric neural crest-derived cells.

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	4F'	Expression was detected in all non-neuronal enteric neural crest-derived cells.

Structure	Level	Pattern	Image	Note
TS20: small intestine	Absent		4I

Structure	Level	Pattern	Image	Note
TS20: small intestine	Present	Regionally restricted	4J	Expression was detected in enteric neural crest-derived cells.

Structure	Level	Pattern	Image	Note
TS20: small intestine	Absent		4K

Structure	Level	Pattern	Image	Note
TS20: lung	Ambiguous		5A

Structure	Level	Pattern	Image	Note
TS20: lung	Ambiguous		5B