Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45211
Buscher D, et al., Expression profile of Gli family members and Shh in normal and mutant mouse limb development. Dev Dyn. 1998 Jan;211(1):88-96
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6160183
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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Gli3 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1 FL E10.5:
embryonic day 10.5
(more  )
Specimen
1 FL E10.5:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Diffuse |
1_Gli3 FL E10.5
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Expression is nearly throughout the whole limb bud except in the most posterior region.
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Specimen
1 FL E11.5:
embryonic day 11.5
(more )
Specimen
1 FL E11.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
1_Gli3 FL E11.5
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Expression is nearly throughout the whole limb bud except in the most posterior region. Patches of higher expression are seen in the central regions.
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Specimen
1 FL E12.5:
embryonic day 12.5
(more )
Specimen
1 FL E12.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1 HL E10.5:
embryonic day 10.5
(more )
Specimen
1 HL E10.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Diffuse |
1_Gli3 HL E10.5
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Expression is nearly throughout the whole limb bud except in the most posterior region.
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Specimen
1 HL E11.5:
embryonic day 11.5
(more )
Specimen
1 HL E11.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
1_Gli3 HL E11.5
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Expression is nearly throughout the whole limb bud except in the most posterior region. Patches of higher expression are seen in the central regions.
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Specimen
1 HL E12.5:
embryonic day 12.5
(more )
Specimen
1 HL E12.5:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
2C:
embryonic day 11.5
(more )
Note: Digitally processed superimposed image is shown at the bottom. Blue - Gli3; red - Shh (assay MGI:6160184).
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Digitally processed superimposed image is shown at the bottom. Blue - Gli3; red - Shh (assay MGI:6160184).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
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2C top left
,
2C bottom
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Expression is strongest in the anterior region, fading gradually towards the posterior part where it is absent.
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Specimen
2D:
embryonic day 11.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
2D bottom
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Expression is strongest in the anterior region, fading gradually towards the posterior part where it is absent.
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Specimen
3 wt:
embryonic day 11.0
(more )
Specimen
3 wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
3_Gli3 wt
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Expression is nearly throughout the whole limb bud except in the most posterior region. Patches of higher expression are seen in the central regions.
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Specimen
3 Xt:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
6 FL Hx:
embryonic day 11.0; Rr29Hx/Rr29 +
(more )
Specimen
6 FL Hx:
(close )
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Genetic Background:
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involves: C57BL/10SnJ * DBA/2J
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Mutant Allele(s):
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Rr29Hx/Rr29+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
6_Gli3 Hx FL
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Expression is throughout except in the most posterior region and in the most anterior region in this mutant.
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Specimen
6 HL Hx:
embryonic day 11.0; Rr29Hx/Rr29 +
(more )
Specimen
6 HL Hx:
(close )
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Genetic Background:
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involves: C57BL/10SnJ * DBA/2J
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Mutant Allele(s):
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Rr29Hx/Rr29+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindlimb bud |
Present |
Regionally restricted |
6_Gli3 Hx HL
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Expression is throughout except in the most posterior region and in the most anterior region in this mutant.
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Specimen
6 FL wt:
embryonic day 11.0
(more )
Specimen
6 FL wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
6_Gli3 wt FL
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Expression is nearly throughout the whole limb bud except in the most posterior region.
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Specimen
6 HL wt:
embryonic day 11.0
(more )
Specimen
6 HL wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindlimb bud |
Present |
Regionally restricted |
6_Gli3 wt HL
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Expression is nearly throughout the whole limb bud except in the most posterior region.
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Specimen
not shown:
embryonic day 9.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: limb |
Present |
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Expression is trhoughout the whole limb bud.
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Analysis Tools