Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		1E Rspo3+/-	Authors report that heterozygous mice developed normally.
TS17: truncus arteriosus	Present		1E Rspo3+/-

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		1E Rspo3-/-	Development of homozygous mice was arrested from E10.5.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	2K	Expression domain surrounding boundary between telencephalon and diencephalon.
TS15: telencephalon	Present	Regionally restricted	2K	Expression domain surrounding boundary between telencephalon and diencephalon.
TS15: neural tube roof plate	Present		2K
TS15: tail	Present		2K

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2L	Expression domain surrounding boundary between telencephalon and diencephalon.
TS19: telencephalon	Present	Regionally restricted	2L	Expression domain surrounding boundary between telencephalon and diencephalon.
TS19: central nervous system roof plate	Present		2L
TS19: head mesenchyme	Present		2L
TS19: otocyst	Present		2L
TS19: somite	Present		2L
TS19: tail	Present		2L
TS19: forelimb bud	Present	Regionally restricted	2L	Expression in limbs was different from pattern of expression of Rspo3 mRNA (assay MGI:6158432).
TS19: hindlimb bud	Present	Regionally restricted	2L	Expression in limbs was different from pattern of expression of Rspo3 mRNA (assay MGI:6158432).

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	2M	Expression domain surrounding boundary between telecephalon and diencephalon.
TS20: telencephalon	Present	Regionally restricted	2M	Expression domain surrounding boundary between telecephalon and diencephalon.
TS20: central nervous system roof plate	Present		2M
TS20: vibrissa	Present		2M
TS20: forelimb	Present	Regionally restricted	2M	Expression in limbs was different from pattern of expression of Rspo3 mRNA (assay MGI:6158432).

Structure	Level	Pattern	Image	Note
TS12: chorion	Weak		3A

Structure	Level	Pattern	Image	Note
TS13: chorioallantoic placenta	Present	Regionally restricted	3B	Expression mainly at edge of fusion interface between chorion and allantois.

Structure	Level	Pattern	Image	Note
TS15: allantois	Present	Regionally restricted	3C	Expression expanded into center of allantois.

Structure	Level	Pattern	Image	Note
TS17: chorioallantoic placenta	Present	Regionally restricted	3D	Expression expanded further; also detected in allantois penetrating into the chorion.

Structure	Level	Pattern	Image	Note
TS12: allantois	Trace		3E	Expression barely detectable in allantois.

Structure	Level	Pattern	Image	Note
TS13: allantois	Present	Regionally restricted	3F	Expression mainly at edge of fusion interface between chorion and allantois.

Structure	Level	Pattern	Image	Note
TS15: allantois	Present	Regionally restricted	3G	Expression mainly at edge of fusion interface between chorion and allantois.
TS15: chorion	Absent		3G	No expression in chorionic trophoblast cells.

Structure	Level	Pattern	Image	Note
TS17: chorioallantoic placenta	Present	Regionally restricted	3H

Structure	Level	Pattern	Image	Note
TS15: allantois	Present		3K
TS15: chorion	Absent		3K	No expression in chorionic trophoblast cells.