Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cone	Present	Regionally restricted	4A	Expression in a central core of the ectoplacental cone.
TS10: embryo	Weak	Widespread	4A	Low levels of expression throughout most of the embryo and extraembryonic tissues.
TS10: extraembryonic component	Weak	Widespread	4A	Low levels of expression throughout most of the embryo and extraembryonic tissues.
TS10: mesoderm	Strong	Regionally restricted	4A	Stronger expression in posterior nascent mesoderm.

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present	Regionally restricted	4B	Expression in a central core of future placental tissue, the ectoplacental cone.
TS11: embryo	Weak	Widespread	4B	Low levels of expression throughout most of the embryo and extraembryonic tissues.
TS11: extraembryonic component	Weak	Widespread	4B	Low levels of expression throughout most of the embryo and extraembryonic tissues.
TS11: mesoderm	Strong	Regionally restricted	4B	Stronger expression in posterior nascent mesoderm.
TS11: blood island	Present		4B

Structure	Level	Pattern	Image	Note
TS13: chorioallantoic placenta	Present	Regionally restricted	4C	Two domains of expression in forming placental region.
TS13: ectoplacental cone	Present	Regionally restricted	4C	Upper expression domain in trophoblast cells of the ectoplacental cone.
TS13: allantois mesenchyme	Present	Regionally restricted	4C	Lower expression domain in mesoderm cells of the allantois, particularly strong in allantoic mesoderm in contact with trophoblasts of chorion.
TS13: chorion	Absent		4C

Structure	Level	Pattern	Image	Note
TS15: chorioallantoic placenta	Present	Regionally restricted	4D	Authors report continuation of expression pattern at 9.5 dpc.
TS15: trophoblast giant cell	Present		4D

Structure	Level	Pattern	Image	Note
TS15: secondary trophoblast giant cell	Present	Regionally restricted	4E	Expression in ectoplacental cone-derived trophoblasts.

Structure	Level	Pattern	Image	Note
TS15: secondary trophoblast giant cell	Present		4F

Structure	Level	Pattern	Image	Note
TS22: yolk sac mesenchyme	Strong		4G	Expression in mesodermal layer of yolk sac, strongly localized to blood vessel walls.

Structure	Level	Pattern	Image	Note
TS23: yolk sac blood vessel	Ambiguous		4H	Authors report difficulty identifying expression in endothelial cells lining blood vessels.

Structure	Level	Pattern	Image	Note
TS22: trophectoderm	Present	Regionally restricted	5A	Expression in all trophoblast-derived layers of the placenta.

Structure	Level	Pattern	Image	Note
TS28: decidua	Absent		5A	No expression in maternally-derived tissue.

Structure	Level	Pattern	Image	Note
TS22: trophectoderm	Strong	Widespread	5B	Strong expression in trophoblast giant cell layer.
TS22: spongiotrophoblast	Very strong		5B	Particularly strong expression in spongiotrophoblast cells.
TS22: labyrinthine zone	Present		5B
TS22: chorioallantoic placenta	Present	Regionally restricted	5B	Expression in endothelial and smooth muscle cells of fetal blood vessels.

Structure	Level	Pattern	Image	Note
TS22: spongiotrophoblast	Present	Regionally restricted	5C	Expression in glycogen cells.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	5C	Expression maintained in glycogen cells as they migrated into the decidua.

Structure	Level	Pattern	Image	Note
TS22: spongiotrophoblast	Present	Regionally restricted	5D	Expression in glycogen cells.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	5D	Expression maintained in glycogen cells as they migrated into the decidua.

Structure	Level	Pattern	Image	Note
TS22: trophoblast giant cell	Strong		5E	Strong expression in trophoblast giant cell layer.
TS22: spongiotrophoblast	Very strong		5E	Particularly strong expression in spongiotrophoblast cells.
TS22: labyrinthine zone	Present		5E
TS22: chorioallantoic placenta	Present	Regionally restricted	5E	Expression in endothelial and smooth muscle cells of fetal blood vessels.

Structure	Level	Pattern	Image	Note
TS22: labyrinthine zone	Present	Regionally restricted	5F	Expression in large fetal vessels at base of labyrinth.
TS22: chorionic plate	Absent		5F	No expression in columnar epithelium at base of labyrinthine layer, the chorionic plate.

Structure	Level	Pattern	Image	Note
TS22: chorionic plate	Absent		5G	No expression in columnar epithelium at base of labyrinthine layer, the chorionic plate.

Structure	Level	Pattern	Image	Note
TS28: decidua	Weak	Regionally restricted	5G	Authors report expression in fewer trophoblast giant cells, spongiotrophoblasts and trophoblast derivatives in the decidua of heterozygous placentas.

Structure	Level	Pattern	Image	Note
TS15: chorioallantoic placenta	Present		7C