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Gene Expression Data
Assay Details
Assay
Reference: J:194961 Meadows SM, et al., Resolution of defective dorsal aortae patterning in Sema3E-deficient mice occurs via angiogenic remodeling. Dev Dyn. 2013 May;242(5):570-80
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6155018
Gene symbol: Emcn
Gene name: endomucin
Antibody: anti-Endomucin
Detection system: Alexa Fluor
Results
Specimen 1B: embryonic day 8.0; Sema3etm1Ddg/Sema3e+ (more )
Note: 7 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS12: blood vessel Present 1B
Specimen 1D: embryonic day 8.0; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 7 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 1D There is aberrant plexus-like vasculature in this mutant.
Specimen 1F: embryonic day 9.5; Sema3etm1Ddg/Sema3e+ (more )
Note: 23 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS15: dorsal aorta Present 1F
Specimen 1H: embryonic day 9.5; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 23 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS15: dorsal aorta Present 1H Expression in this mutant is the same as in wild-type embryos.
Specimen 1I: postnatal adult; Sema3etm1Ddg/Sema3e+ (more )
Note: Double labeled: green - Emcn and Pecam1 (assay MGI:6155001); red - Acta2 (assay MGI:6155014).
Structure Level Pattern Image Note
TS28: aorta endothelium Present 1I PECAM+Endomucin , 1I merge
TS28: descending aorta Present 1I PECAM+Endomucin , 1I merge
Specimen 1J: postnatal adult; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: Double labeled: green - Emcn and Pecam1 (assay MGI:6155001); red - Acta2 (assay MGI:6155014).
Structure Level Pattern Image Note
TS28: aorta endothelium Present 1J PECAM+Endomucin , 1J merge Expression is the same as in wild-type.
TS28: descending aorta Present 1J PECAM+Endomucin , 1J merge Expression is the same as in wild-type.
Specimen 3A: embryonic day 8.5; Sema3etm1Ddg/Sema3e+ (more )
Note: 9 somite stage embryo. Double labeled: red - Emcn and Pecam1 (assay MGI:6155001); green - phospho-histone.
Structure Level Pattern Image Note
TS13: dorsal aorta Present 3A
Specimen 3B: embryonic day 8.5; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 9 somite-stage embryo. Double labeled: red - Emcn and Pecam1 (assay MGI:6155001); green - phospho-histone.
Structure Level Pattern Image Note
TS13: dorsal aorta Present 3B Expression in this mutant is the same as in wild-type embryos.
Specimen 3D: embryonic day 8.5; Sema3etm1Ddg/Sema3e+ (more )
Note: 7 somite stage embryo. Double labeled: red - Emcn and Pecam1 (assay MGI:6155001); green - Casp3 (assay MGI:6155016).
Structure Level Pattern Image Note
TS13: dorsal aorta Present 3D
Specimen 3E: embryonic day 8.5; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 7 somite stage embryo. Double labeled: red - Emcn and Pecam1 (assay MGI:6155001); green - Casp3 (assay MGI:6155016).
Structure Level Pattern Image Note
TS13: dorsal aorta Present 3E Expression in this mutant is the same as in wild-type embryos.
Specimen 3G: embryonic day 8.0; Sema3etm1Ddg/Sema3e+ (more )
Note: 7 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 3G , 3G'
Specimen 3H: embryonic day 8.0; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 7 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 3H , 3H' Expression in this mutant is the same as in wild-type embryos.
Specimen 6A: embryonic day 8.0; Plxnd1tm1Joe/Plxnd1+ (more )
Note: 7 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 6A
Specimen 6B: embryonic day 8.0; Plxnd1tm1Joe/Plxnd1tm1Joe (more )
Note: 7 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 6B The mutant dorsal aorta has a branched, disorganized, and plexus-like appearance compared to wild-type.
Specimen 6C: embryonic day 8.0; Plxnd1tm1Joe/Plxnd1+ (more )
Note: 8 somite stage embryo.Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 6C
Specimen 6D: embryonic day 8.0; Plxnd1tm1Joe/Plxnd1tm1Joe (more )
Note: 8 somite stage embryo. Also stained for Pecam1 (assay MGI:6155001); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 6D The mutant dorsal aorta has a branched, disorganized, and plexus-like appearance compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory