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Gene Expression Data
Assay Details
Assay
Reference: J:194961 Meadows SM, et al., Resolution of defective dorsal aortae patterning in Sema3E-deficient mice occurs via angiogenic remodeling. Dev Dyn. 2013 May;242(5):570-80
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6155001
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-PECAM
Detection system: Alexa Fluor
Results
Specimen 1A: embryonic day 8.0; Sema3etm1Ddg/Sema3e+ (more )
Note: 7 somite stage embryo.
Structure Level Pattern Image Note
TS12: blood vessel Present 1A
TS12: dorsal aorta Present 1A
Specimen 1B: embryonic day 8.0; Sema3etm1Ddg/Sema3e+ (more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS12: blood vessel Present 1B
Specimen 1C: embryonic day 8.0; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 7 somite stage embryo.
Structure Level Pattern Image Note
TS12: blood vessel Present 1C There is aberrant plexus-like vasculature in this mutant.
TS12: dorsal aorta Present 1C There is aberrant plexus-like vasculature in this mutant.
Specimen 1D: embryonic day 8.0; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 1D There is aberrant plexus-like vasculature in this mutant.
Specimen 1F: embryonic day 9.5; Sema3etm1Ddg/Sema3e+ (more )
Note: 23 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS15: dorsal aorta Present 1F
Specimen 1H: embryonic day 9.5; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 23 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS15: dorsal aorta Present 1H Expression in this mutant is the same as in wild-type embryos.
Specimen 1I: postnatal adult; Sema3etm1Ddg/Sema3e+ (more )
Note: Double labeled: green - Pecam1 and Emcn (assay MGI:6155018); red - Acta2 (assay MGI:6155014).
Structure Level Pattern Image Note
TS28: aorta endothelium Present 1I merge , 1I PECAM+Endomucin
TS28: descending aorta Present 1I merge , 1I PECAM+Endomucin
Specimen 1J: postnatal adult; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: Double labeled: green - Pecam1 and Emcn (assay MGI:6155018); red - Acta2 (assay MGI:6155014).
Structure Level Pattern Image Note
TS28: aorta endothelium Present 1J merge , 1J PECAM+Endomucin Expression is the same as in wild-type.
TS28: descending aorta Present 1J merge , 1J PECAM+Endomucin Expression is the same as in wild-type.
Specimen 3A: embryonic day 8.5; Sema3etm1Ddg/Sema3e+ (more )
Note: 9 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - phospho-histone.
Structure Level Pattern Image Note
TS13: dorsal aorta Present 3A
Specimen 3B: embryonic day 8.5; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 9 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - phospho-histone.
Structure Level Pattern Image Note
TS13: dorsal aorta Present 3B Expression in this mutant is the same as in wild-type embryos.
Specimen 3D: embryonic day 8.5; Sema3etm1Ddg/Sema3e+ (more )
Note: 7 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - Casp3 (assay MGI:6155016).
Structure Level Pattern Image Note
TS13: dorsal aorta Present 3D
Specimen 3E: embryonic day 8.5; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 7 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - Casp3 (assay MGI:6155016).
Structure Level Pattern Image Note
TS13: dorsal aorta Present 3E Expression in this mutant is the same as in wild-type embryos.
Specimen 3G: embryonic day 8.0; Sema3etm1Ddg/Sema3e+ (more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 3G , 3G'
Specimen 3H: embryonic day 8.0; Sema3etm1Ddg/Sema3etm1Ddg (more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 3H' , 3H Expression in this mutant is the same as in wild-type embryos.
Specimen 6A: embryonic day 8.0; Plxnd1tm1Joe/Plxnd1+ (more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 6A
Specimen 6B: embryonic day 8.0; Plxnd1tm1Joe/Plxnd1tm1Joe (more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 6B The mutant dorsal aorta has a branched, disorganized, and plexus-like appearance compared to wild-type.
Specimen 6C: embryonic day 8.0; Plxnd1tm1Joe/Plxnd1+ (more )
Note: 8 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 6C
Specimen 6D: embryonic day 8.0; Plxnd1tm1Joe/Plxnd1tm1Joe (more )
Note: 8 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Structure Level Pattern Image Note
TS12: dorsal aorta Present 6D The mutant dorsal aorta has a branched, disorganized, and plexus-like appearance compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory