Gene Expression Data
Assay Details
Assay
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Reference:
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J:194961
Meadows SM, et al., Resolution of defective dorsal aortae patterning in Sema3E-deficient mice occurs via angiogenic remodeling. Dev Dyn. 2013 May;242(5):570-80
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6155001
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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anti-PECAM
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Detection system:
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Alexa Fluor
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Results
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Specimen
1A:
embryonic day 8.0; Sema3etm1Ddg/Sema3e +
(more )
Note: 7 somite stage embryo.
Specimen
1A:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
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Mutant Allele(s):
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Sema3etm1Ddg/Sema3e+
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Age:
|
embryonic day 8.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somite stage embryo.
|
Specimen
1B:
embryonic day 8.0; Sema3etm1Ddg/Sema3e +
(more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Specimen
1B:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
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Mutant Allele(s):
|
Sema3etm1Ddg/Sema3e+
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Specimen
1C:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
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Mutant Allele(s):
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Sema3etm1Ddg/Sema3etm1Ddg
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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7 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: blood vessel |
Present |
|
1C
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There is aberrant plexus-like vasculature in this mutant.
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TS12: dorsal aorta |
Present |
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1C
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There is aberrant plexus-like vasculature in this mutant.
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Specimen
1D:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
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Mutant Allele(s):
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Sema3etm1Ddg/Sema3etm1Ddg
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: dorsal aorta |
Present |
|
1D
|
There is aberrant plexus-like vasculature in this mutant.
|
Specimen
1F:
embryonic day 9.5; Sema3etm1Ddg/Sema3e +
(more )
Note: 23 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Specimen
1F:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * CD-1
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Mutant Allele(s):
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Sema3etm1Ddg/Sema3e+
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
23 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Specimen
1H:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
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Mutant Allele(s):
|
Sema3etm1Ddg/Sema3etm1Ddg
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
23 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: dorsal aorta |
Present |
|
1H
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
1I:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * CD-1
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Mutant Allele(s):
|
Sema3etm1Ddg/Sema3e+
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
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Double labeled: green - Pecam1 and Emcn (assay MGI:6155018); red - Acta2 (assay MGI:6155014).
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Specimen
1J:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * CD-1
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Mutant Allele(s):
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Sema3etm1Ddg/Sema3etm1Ddg
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Pecam1 and Emcn (assay MGI:6155018); red - Acta2 (assay MGI:6155014).
|
Specimen
3A:
embryonic day 8.5; Sema3etm1Ddg/Sema3e +
(more )
Note: 9 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - phospho-histone.
Specimen
3A:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Sema3etm1Ddg/Sema3e+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
9 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - phospho-histone.
|
Specimen
3B:
embryonic day 8.5; Sema3etm1Ddg/ Sema3etm1Ddg
(more )
Note: 9 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - phospho-histone.
Specimen
3B:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Sema3etm1Ddg/Sema3etm1Ddg
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
9 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - phospho-histone.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: dorsal aorta |
Present |
|
3B
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
3D:
embryonic day 8.5; Sema3etm1Ddg/Sema3e +
(more )
Note: 7 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - Casp3 (assay MGI:6155016).
Specimen
3D:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Sema3etm1Ddg/Sema3e+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
7 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - Casp3 (assay MGI:6155016).
|
Specimen
3E:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Sema3etm1Ddg/Sema3etm1Ddg
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
7 somite stage embryo. Double labeled: red - Pecam1 and Emcn (assay MGI:6155018); green - Casp3 (assay MGI:6155016).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: dorsal aorta |
Present |
|
3E
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
3G:
embryonic day 8.0; Sema3etm1Ddg/Sema3e +
(more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Specimen
3G:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Sema3etm1Ddg/Sema3e+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Specimen
3H:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6 * CD-1
|
Mutant Allele(s):
|
Sema3etm1Ddg/Sema3etm1Ddg
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: dorsal aorta |
Present |
|
3H'
,
3H
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
6A:
embryonic day 8.0; Plxnd1tm1Joe/Plxnd1 +
(more )
Note: 7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Specimen
6A:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
|
Plxnd1tm1Joe/Plxnd1+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Specimen
6B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
|
Plxnd1tm1Joe/Plxnd1tm1Joe
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
7 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: dorsal aorta |
Present |
|
6B
|
The mutant dorsal aorta has a branched, disorganized, and plexus-like appearance compared to wild-type.
|
Specimen
6C:
embryonic day 8.0; Plxnd1tm1Joe/Plxnd1 +
(more )
Note: 8 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
Specimen
6C:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Plxnd1tm1Joe/Plxnd1+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
8 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Specimen
6D:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Plxnd1tm1Joe/Plxnd1tm1Joe
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
8 somite stage embryo. Also stained for Emcn (assay MGI:6155018); both green.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: dorsal aorta |
Present |
|
6D
|
The mutant dorsal aorta has a branched, disorganized, and plexus-like appearance compared to wild-type.
|
|