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Gene Expression Data
Assay Details
Assay
Reference: J:198558 Li W, et al., Tissue-specific venous expression of the EPH family receptor EphB1 in the skin vasculature. Dev Dyn. 2013 Aug;242(8):976-88
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6150916
Gene symbol: Ephb4
Gene name: Eph receptor B4
Reporter: lacZ
Antibody: anti-B-galactosidase (0855976)
Detection system: Secondary antibody
Results
Specimen 1A/C/D: embryonic day 15.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: green - Ephb4; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: vein Present 1A/A'/A" , 1D/D'/D" , 1C/C'/C" Mainly detected in large-diameter veins and some branched veins.
TS23: lymphatic vessel Weak 1C/C'/C" , 1A/A'/A" , 1D/D'/D"
Specimen 4A/G/J: embryonic day 13.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: green - Ephb4; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS21: vascular plexus Present Regionally restricted 4A , 4G , 4J Expressed in capillary plexus.
Specimen 4B/H/K: embryonic day 14.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: green - Ephb4; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS22: vein Present 4K , 4B , 4H Weak or faint expression in some smaller vessels.
TS22: arterial blood vessel Present 4H , 4K , 4B Expression was progressively decreased.
Specimen 4C/I/L: embryonic day 15.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: green - Ephb4; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: lymphatic vessel Present 4L , 4C , 4I
TS23: vein Present 4L , 4C , 4I
Specimen 7A/G: postnatal adult; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: green - Ephb4; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS28: lymphatic vessel Present 7A , 7G
TS28: vein Present 7A , 7G
TS28: artery Absent 7G , 7A
Specimen 8C: embryonic day 13.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: green - Ephb4; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS21: cardinal vein Present 8C
TS21: lymphatic system Present 8C
Specimen 9A: embryonic day 8.0; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: green - Ephb4; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 9A Expressed in capillary networks.
Specimen 9E/G: embryonic day 15.5; Ephb4tm1And/Ephb4+ (more )
Note: Triple labeled: green - Ephb4; red - Ephb1 (assay MGI:6150925); blue - Pecam1 (assay MGI:6150928).
Structure Level Pattern Image Note
TS23: lung vascular element Present 9E , 9G Expressed in lung microvasculature.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory