Nodal RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:252050 Sung YH, et al., PIERCE1 is critical for specification of left-right asymmetry in mice. Sci Rep. 2016 Jun 16;6:27932
Assay type:	RNA in situ
MGI Accession ID:	MGI:6147599
Gene symbol:	Nodal
Gene name:	nodal growth differentiation factor

Probe:	Nodal probe27
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

2A1 ©

Specimen 2A1: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	2A1	Expressed on the left side.

2A2 ©

Specimen 2A2: embryonic day 8.0; Pierce1^{Gt(OST3440)Lex}/Pierce1^{Gt(OST3440)Lex} (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	2A2	Ramdomized expression. In 4 out of 9 embryos expressed in the left side.

2A3 ©

Specimen 2A3: embryonic day 8.0; Pierce1^{Gt(OST3440)Lex}/Pierce1^{Gt(OST3440)Lex} (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	2A3	Ramdomized expression. In 1 out of 9 embryos expressed in the right side.

2A4 ©

Specimen 2A4: embryonic day 8.0; Pierce1^{Gt(OST3440)Lex}/Pierce1^{Gt(OST3440)Lex} (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted	2A4	Ramdomized expression. In 1 out of 9 embryos expressed in both sides.

2A5 ©

Specimen 2A5: embryonic day 8.0; Pierce1^{Gt(OST3440)Lex}/Pierce1^{Gt(OST3440)Lex} (more

)

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent		2A5	Ramdomized expression. In 3 out of 9 embryos had no expression.

S4_left ©

Specimen S4 left: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	S4_left	Expressed in the perinodal cells.

S4_middle ©

Specimen S4 middle: embryonic day 8.0; Pierce1^{Gt(OST3440)Lex}/Pierce1^{Gt(OST3440)Lex} (more

)

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	S4_middle	Expressed in the perinodal cells, same as in the control.

S4_right ©

Specimen S4 right: embryonic day 8.0; Pierce1^{Gt(OST3440)Lex}/Pierce1^{Gt(OST3440)Lex} (more

)

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	S4_right	Expressed in the perinodal cells, same as in the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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