Gene Expression Data
Assay Details
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Assay
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Reference:
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J:33225
Wu JE, et al., Differential expression of integrin alpha subunits supports distinct roles during lung branching morphogenesis. Dev Dyn. 1996 Jun;206(2):169-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6147344
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Gene symbol:
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Itga2
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Gene name:
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integrin alpha 2
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Antibody:
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MA2 antibody
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
not shown:
embryonic day 10.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: lung mesenchyme |
Present |
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| TS17: lung epithelium |
Present |
Regionally restricted |
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Expression was observed only on a subset of epithelial cells. Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips.
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Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung mesenchyme |
Present |
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| TS24: lung epithelium |
Present |
Regionally restricted |
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Expression was observed only on a subset of epithelial cells. Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips.
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Specimen
2F:
embryonic day 14.5
(more )
Specimen
2F:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung mesenchyme |
Present |
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2F
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Most mesenchymal cells expressed Itga2 and Itga6 concomitantly.
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| TS22: lung epithelium |
Present |
Regionally restricted |
2F
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Expression was observed only on a subset of epithelial cells.
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Specimen
4C:
embryonic day 14.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
Non-Uniform |
4C
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Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips.
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Specimen
4F:
embryonic day 14.5
(more )
Specimen
4F:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
Non-Uniform |
4F
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Expression was high at branch tips and markedly decreased to an undetectable level distal to the tips.
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Specimen
6C:
embryonic day 12.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lung mesenchyme |
Present |
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6C
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| TS20: lung epithelium |
Present |
Non-Uniform |
6C
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Expression was restricted to tips of the epithelial tubules, areas displaying high levels of Collagen IV mRNA. Expression on more mature central airway epithelium was greatly diminished to undetectable.
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Specimen
7B:
embryonic day 15.0
(more )
Specimen
7B:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung mesenchyme |
Present |
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7B
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| TS23: lung epithelium |
Present |
Regionally restricted |
7B
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Expression was detected in epithelia that were positive for Lamb1 mRNA. Expression in more central airways was undetectable.
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Specimen
7E:
embryonic day 14.5
(more )
Specimen
7E:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung mesenchyme |
Present |
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7E
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| TS22: lung epithelium |
Present |
Regionally restricted |
7E
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Expression was detected in epithelia that were positive for Lamc1 mRNA. Expression in more central airways was undetectable.
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Specimen
7H:
embryonic day 14.5
(more )
Specimen
7H:
(close )
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Genetic Background:
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involves: BALB/c * C3H
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung mesenchyme |
Present |
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7H
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| TS22: lung epithelium |
Present |
Regionally restricted |
7H
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Expression was detected in epithelia that were positive for Lama1 mRNA. Expression in areas lacking Lama1 mRNA was undetectable.
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