Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:88779
Martin DM, et al., PITX2 is required for normal development of neurons in the mouse subthalamic nucleus and midbrain. Dev Biol. 2004 Mar 1;267(1):93-108
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6118165
|
|
Gene symbol:
|
Pitx2
|
|
Gene name:
|
paired-like homeodomain transcription factor 2
|
|
Probe:
|
Pitx2 cDNA6
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
2A:
embryonic day 14.5
(more  )
Specimen
2A:
(close  )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
2A
|
Expression near pial surface.
|
Specimen
2B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
2B
|
Authors report a distinct difference in the pattern of expression between wild-type and mutant embryos.
|
Specimen
2C:
embryonic day 14.5
(more )
Specimen
2C:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
2C
|
Expression in nuclei of cells in superficial mesencephalon.
|
Specimen
2D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
2D
|
Expression only in deeper layers of the neuroepithelium.
|
| TS22: superior colliculus |
Present |
Regionally restricted |
2D
|
Expression displaced toward the intermediate and subventricular zone of the developing superior colliculus.
|
Specimen
2E:
embryonic day 14.5
(more )
Specimen
2E:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
2E
|
Expression in nuclei of cells in superficial mesencephalon.
|
Specimen
2F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
2F
|
Expression only in deeper layers of the neuroepithelium.
|
| TS22: superior colliculus |
Present |
Regionally restricted |
2F
|
Expression displaced toward the intermediate and subventricular zone of the developing superior colliculus.
|
Specimen
2G:
embryonic day 13.5
(more )
Specimen
2G:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
2G
|
Expression in outermost differentiating region of lateral dorsal midbrain and in more medial locations.
|
Specimen
2H:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: midbrain |
Present |
Regionally restricted |
2H
|
Expression absent from outermost differentiating region of lateral dorsal midbrain. Expression preserved in more medial locations.
|
Specimen
2I:
embryonic day 14.5
(more )
Specimen
2I:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2J:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: zona limitans intrathalamica |
Present |
|
2J
|
Authors state that the location and number of expressing neurons in the zona limitans are unchanged in mutant embryos compared to wild-type.
|
Specimen
E14.5 wt not shown:
embryonic day 14.5
(more )
Specimen
E14.5 wt not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
E14.5 -/- not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mammillary area |
Present |
|
|
Authors report normal expression in mammillary region.
|
| TS22: hindbrain |
Present |
Regionally restricted |
|
Authors report normal expression in first rhombomere.
|
Specimen
4A:
embryonic day 14.5
(more )
Specimen
4A:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
4A
|
Expression in subthalamic nucleus.
|
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
4B
|
Authors report loss of expression in subthalamic nucleus. Accumulation of expression in intermediate and subventricular zones of the ventrolateral thalamic neuroepithelium.
|
Specimen
4C:
embryonic day 14.5
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
4C
|
Expression in subthalamic nucleus.
|
Specimen
4D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
4D
|
Authors report loss of expression in the ventrolateral thalamus.
|
Specimen
4E:
embryonic day 13.5
(more )
Specimen
4E:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: prethalamus |
Present |
Regionally restricted |
4E
|
Expression in subthalamic nucleus. Expression in the lateral-most aspect of the thalamus, with denser expression nearer the ventricle.
|
Specimen
4F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: prethalamus |
Present |
Regionally restricted |
4F
|
Authors report loss of expression in the lateral-most aspect of the thalamus. Continued denser expression nearer the ventricle.
|
Specimen
E12.5 wt not shown:
embryonic day 12.5
(more )
Specimen
E12.5 wt not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: prethalamus |
Present |
Regionally restricted |
|
Expression in ventrolateral thalamus.
|
Specimen
E12.5 -/- not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: prethalamus |
Present |
Regionally restricted |
|
Authors state expression patterns in ventrolateral thalamus was similar to wild-type at E12.5 and earlier.
|
Specimen
5B:
embryonic day 14.5
(more )
Specimen
5B:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
5B
|
Expression in subthalamic nucleus.
|
Specimen
5C:
embryonic day 14.5
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
5C
|
Expression in subthalamic nucleus.
|
Specimen
E16.5 neo/- not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm1Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: prethalamus |
Present |
Regionally restricted |
|
Authors report preservation of expression in the subthalamic nucleus of E16.5 neo/- embryos.
|
Specimen
E18.5 neo/neo not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm1Sac/Pitx2tm1Sac
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: prethalamus |
Present |
Regionally restricted |
|
Authors report preservation of expression in the subthalamic nucleus of E18.5 neo/neo embryos.
|
Specimen
7A:
embryonic day 14.5
(more )
Specimen
7A:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
7A
|
Expression in subthalamic nucleus.
|
Specimen
7B:
embryonic day 14.5; Pitx2tm2Sac/Pitx2 +
(more )
Specimen
7B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
7B
|
Expression in subthalamic nucleus.
|
Specimen
7C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm1Sac/Pitx2tm1Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
7C
|
Expression in subthalamic nucleus.
|
Specimen
7D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm1Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
8A:
embryonic day 14.5
(more )
Specimen
8A:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
8A
|
Expression in ventrolateral thalamus.
|
Specimen
8B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Pitx2tm2Sac/Pitx2tm2Sac
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: prethalamus |
Present |
Regionally restricted |
8B
|
No expression in ventrolateral thalamus.
|
|
Analysis Tools