Antibody:	anti-HGF
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: oral region	Moderate	Regionally restricted	1a	Expressed diffusely in the bilateral oval area containing mesenchymal cells beneath the oral epithelium.

Structure	Level	Pattern	Image	Note
TS20: tongue mesenchyme	Moderate	Regionally restricted	2a	No longer present in the round mesenchymal cells but weak to moderate intensity in the long spindle-like cells arranged radially and longitudinally to the lingual surface from the dorsal pharyngeal area. Colocalized with Met in the myogenic cells.

Structure	Level	Pattern	Image	Note
TS22: tongue intrinsic muscle	Strong	Diffuse	2d	Colocalized with Met and Des.

Structure	Level	Pattern	Image	Note
TS24: tongue intrinsic muscle	Present		2g	Expression was markedly reduced.

Structure	Level	Pattern	Image	Note
TS19: oral region mesenchyme	Present	Diffuse		Expressed in the mesenchymal cells, which were round in shape and distributed diffusely in the floor of the oral cavity.