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Gene Expression Data
Assay Details
Assay
Reference: J:235365 Yzaguirre AD, et al., Insights into blood cell formation from hemogenic endothelium in lesser-known anatomic sites. Dev Dyn. 2016 Oct;245(10):1011-28
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6117870
Gene symbol: Runx1
Gene name: runt related transcription factor 1
Antibody: anti-RUNX1/AML1 + RUNX2 + RUNX3 (EPR3099; ab92336)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A: embryonic day 8.0 (more )
Note: Confocal Z-projection. Late headfold stage embryo. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 1Aii Expression is in a band in the endothelial network and in small populations distal to the band.
Specimen 1B: embryonic day 8.0 (more )
Note: Confocal Z-projection. Late headfold stage embryo. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 1Bii
Specimen 1C: embryonic day 8.0 (more )
Note: Single optical projection. Late headfold stage embryo. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 1Cii
Specimen 3B: embryonic day 9.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 3Bii Expression is in the proximal region of the vascular plexus in close proximity of the vitelline artery.
Specimen 3C: embryonic day 9.5 (more )
Note: Single optical projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 3Cii Expression is in the proximal region of the vascular plexus in close proximity of the vitelline artery.
Specimen 3D: embryonic day 9.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 3Dii Expression is in the proximal region of the vascular plexus in close proximity of the vitelline vein.
Specimen 3E: embryonic day 9.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 3Eii Expression is in the proximal region of the vascular plexus in close proximity of the vitelline vein.
Specimen 3G: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 3Gii
Specimen 3H: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 3Hii
Specimen 3I: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 3Jii , 3Iii
Specimen 6A: embryonic day 8.0 (more )
Note: Confocal Z-projection. Late headfold stage embryo. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS12: blood vessel Present Regionally restricted 6Aii Expression is in the vascular intersection among the placenta, yolk sac, and embryo.
TS12: yolk sac Present Regionally restricted 6Aii Expression is in the vascular intersection among the placenta, yolk sac, and embryo.
TS28: placenta Present Regionally restricted 6Aii Expression is in the vascular intersection among the placenta, yolk sac, and embryo.
TS12: primordial germ cell of yolk sac Absent 6Aii
Specimen 7A: embryonic day 9.5 (more )
Note: Confocal Z-projection. 25 somite pair embryo. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: dorsal aorta Present Regionally restricted 7Aii , 7Ai Expression is in the endothelium.
TS15: umbilical artery embryonic part Present Regionally restricted 7Aii , 7Ai Expression is in the endothelium.
TS15: vitelline artery Present Regionally restricted 7Aii , 7Ai Expression is in the endothelium.
Specimen 7B: embryonic day 9.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: dorsal aorta Present Regionally restricted 7Bi , 7Bii Expression is in the endothelium.
TS15: vitelline artery Present Regionally restricted 7Bi , 7Bii Expression is in the endothelium.
Specimen 7C: embryonic day 9.5 (more )
Note: Single optical projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: dorsal aorta Present Regionally restricted 7C Expression is in the endothelium.
TS15: vitelline artery Present Regionally restricted 7C Expression is in the endothelium.
Specimen 7D: embryonic day 9.5 (more )
Note: Single optical projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: vitelline artery Present Regionally restricted 7D Expression is in the endothelium.
Specimen 8A: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: umbilical artery embryonic part Present Regionally restricted 8Aii Expression is in hematopoietic clusters.
Specimen 8B: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: umbilical artery embryonic part Present Regionally restricted 8Bii Expression is in hematopoietic clusters.
Specimen 8C: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 8Cii Expression is in hematopoietic clusters.
Specimen 8D: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 8Dii Expression is in hematopoietic clusters.
Specimen 9Aii E8.5: embryonic day 8.5 (more )
Note: Confocal Z-projection. 9 somite pair embryo. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS13: common atrial chamber Present Single cells 9Aii E8.5 Expression is in a few dispersed cells and is absent in the endocardium.
TS13: primitive ventricle Present Single cells 9Aii E8.5 Expression is in a few dispersed cells and is absent in the endocardium.
Specimen 9Aii E9.0: embryonic day 9.0 (more )
Note: Confocal Z-projection. 16 somite pair embryo. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS14: common atrial chamber Present Regionally restricted 9Aii E9.0 Expression is in a few dispersed cells and is absent in the endocardium.
TS14: primitive ventricle Present Regionally restricted 9Aii E9.0 Expression is in a few dispersed cells and is absent in the endocardium.
Specimen 9Aii E9.5: embryonic day 9.5 (more )
Note: Confocal Z-projection. 22 somite pair embryo. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS15: heart atrium Present Regionally restricted 9Aii E9.5 Expression is in a few dispersed cells.
TS15: primitive ventricle Present Regionally restricted 9Aii E9.5 Expression is in a few dispersed cells.
TS15: endocardial lining Absent 9Aii E9.5
Specimen 9B: embryonic day 10.5 (more )
Note: Confocal Z-projection. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present Single cells 9B
Specimen 9C: embryonic day 10.5 (more )
Note: Confocal Z-projection. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS17: atrioventricular canal endocardial lining Present Single cells 9C
Specimen 9E: embryonic day 10.5 (more )
Note: Confocal Z-projection. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS17: atrioventricular canal Present Clusters 9E
Specimen 9F top: embryonic day 11.5 (more )
Note: Confocal Z-projection. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present Regionally restricted 9F Expression is in hematopoietic cells from cardiac blood islands that balloon from the ventricular endocardium.
Specimen 9F bottom left: embryonic day 11.5 (more )
Note: Confocal Z-projection. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present Regionally restricted 9F bottom left Expression is in hematopoietic cells from cardiac blood islands that balloon from the ventricular endocardium.
Specimen 9F bottom right: embryonic day 11.5 (more )
Note: Confocal Z-projection. Double labeled: white - Runx1; red - Pecam1 (assay MGI:6117749).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present Regionally restricted 9F bottom right Expression is in hematopoietic cells from cardiac blood islands that balloon from the ventricular endocardium.
Specimen 10A: embryonic day 9.5 (more )
Note: Confocal Z-projection. B: Magnified view of the boxed region in (A). Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: blood vessel Present Scattered 10Bii , 10Aii
TS15: vascular plexus Present Scattered 10Bi , 10Ai Expression is in the cephalic vascular plexus.
Specimen 10C: embryonic day 10.0 (more )
Note: Confocal Z-projection. D: Magnified view of the boxed region in (C). Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS16: vascular plexus Present Regionally restricted 10Ci , 10Cii , 10Di , 10Dii Expression is in cells located at the periphery of the cephalic plexus.
Specimen 10E: embryonic day 10.5 (more )
Note: Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).Confocal Z-projection.
Structure Level Pattern Image Note
TS17: vascular plexus Present Regionally restricted 10Ei , 10Eii , 10Fii Expression is in small clusters located at the periphery of the cephalic plexus.
TS17: internal carotid artery Absent 10Eii , 10Fi , 10Fii
Specimen 10G: embryonic day 10.5 (more )
Note: Confocal Z-projection. H: Magnified view of boxed region in (G). Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: vascular plexus Present Regionally restricted 10Hii , 10Gi Expression is in the cephalic vascular plexus.
TS17: carotid artery Present Regionally restricted 10Gi , 10Hi
Specimen 11A: embryonic day 10.5 (more )
Note: Confocal Z-projection. B: Magnified view of the hematopoietic cluster shown in (A). Triple labeled: white - Runx1; red - Pecam1 (assay MGI:6117749); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: intersomitic blood vessel Present Regionally restricted 11Bii , 11Aii Expression is in hematopietic clusters.
TS17: blood vessel Present Regionally restricted 11Aii , 11Bii Expression is in hematopietic clusters in the dorsal longitudinal anastomotic vessel.
Specimen not shown E7.5: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: blood island Present
Specimen not shown E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: umbilical vein Absent
TS15: vitelline vein Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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