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Gene Expression Data
Assay Details
Assay
Reference: J:235365 Yzaguirre AD, et al., Insights into blood cell formation from hemogenic endothelium in lesser-known anatomic sites. Dev Dyn. 2016 Oct;245(10):1011-28
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6117749
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 1A: embryonic day 8.0 (more )
Note: Confocal Z-projection. Late headfold stage embryo. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 1Ai Expression is in the endothelium.
Specimen 1B: embryonic day 8.0 (more )
Note: Confocal Z-projection. Late headfold stage embryo. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 1Bi Expression is in the endothelium.
Specimen 1C: embryonic day 8.0 (more )
Note: Single optical projection. Late headfold stage embryo. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 1Ci Expression is in the endothelium.
Specimen 2C Arterial: embryonic day 10.5 (more )
Note: Confocal Z-projection.
Structure Level Pattern Image Note
TS17: vitelline artery Present Regionally restricted 2C Arterial Expression is in the endothelium.
TS17: yolk sac blood vessel Present Regionally restricted 2C Arterial Expression is in the endothelium.
Specimen 2C Venous: embryonic day 10.5 (more )
Note: Confocal Z-projection.
Structure Level Pattern Image Note
TS17: vitelline vein Present Regionally restricted 2C Venous Expression is in the endothelium.
TS17: yolk sac blood vessel Present Regionally restricted 2C Venous Expression is in the endothelium.
Specimen 3A: embryonic day 9.5 (more )
Note: Confocal Z-projection. 22 somite pair embryo. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: vitelline artery Present Regionally restricted 3A Expression is in the endothelium.
TS15: vitelline vein Present Regionally restricted 3A Expression is in the endothelium.
TS15: yolk sac blood vessel Present Regionally restricted 3A Expression is in the endothelium.
Specimen 3B: embryonic day 9.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: vitelline artery Present Regionally restricted 3Bi Expression is in the endothelium.
Specimen 3C: embryonic day 9.5 (more )
Note: Single optical projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 3Ci Expression is in the endothelium.
Specimen 3D: embryonic day 9.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 3Di Expression is in the endothelium.
Specimen 3E: embryonic day 9.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 3Ei Expression is in the endothelium.
Specimen 3F: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: vitelline artery Present Regionally restricted 3F Expression is in the endothelium.
TS17: vitelline vein Present Regionally restricted 3F Expression is in the endothelium.
TS17: yolk sac blood vessel Present Regionally restricted 3F Expression is in the endothelium.
Specimen 3G: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 3Gi Expression is in the endothelium.
Specimen 3H: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 3Hi Expression is in the endothelium.
Specimen 3I: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 3Ji , 3Ii Expression is in the endothelium.
Specimen 6A: embryonic day 8.0 (more )
Note: Confocal Z-projection. Late headfold stage embryo. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS12: blood vessel Present Regionally restricted 6Ai Expression is in the endothelium.
TS12: yolk sac Present Regionally restricted 6Ai Expression is in the endothelium.
TS28: placenta Present Regionally restricted 6Ai Expression is in the endothelium.
TS12: primordial germ cell of yolk sac Present 6Ai
Specimen 7A: embryonic day 9.5 (more )
Note: Confocal Z-projection. 25 somite pair embryo. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: blood vessel Present Regionally restricted 7Ai Expression is in the endothelium.
TS15: dorsal aorta Present Regionally restricted 7Ai Expression is in the endothelium.
TS15: umbilical artery embryonic part Present Regionally restricted 7Ai Expression is in the endothelium.
TS15: vitelline artery Present Regionally restricted 7Ai Expression is in the endothelium.
TS15: vitelline vein Present Regionally restricted 7Ai Expression is in the endothelium.
Specimen 7B: embryonic day 9.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: dorsal aorta Present Regionally restricted 7Bi , 7Bi right panel Expression is in the endothelium.
TS15: vitelline artery Present Regionally restricted 7Bi , 7Bi right panel Expression is in the endothelium.
TS15: primordial germ cell Present 7Bi , 7Bi right panel
Specimen 7C: embryonic day 9.5 (more )
Note: Single optical projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: dorsal aorta Present Regionally restricted 7C Expression is in the endothelium.
TS15: vitelline artery Present Regionally restricted 7C Expression is in the endothelium.
Specimen 7D: embryonic day 9.5 (more )
Note: Single optical projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: vitelline artery Present Regionally restricted 7D Expression is in the endothelium.
Specimen 8A: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: umbilical artery embryonic part Present Regionally restricted 8Ai Expression is in the endothelium.
Specimen 8B: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: umbilical artery embryonic part Present Regionally restricted 8Bi Expression is in the endothelium.
Specimen 8C: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 8Ci Expression is in the endothelium.
Specimen 8D: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present Regionally restricted 8Di Expression is in the endothelium.
Specimen 9Ai E8.5: embryonic day 8.5 (more )
Note: Confocal Z-projection. 9 somite pair embryo. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS13: common atrial chamber Present Regionally restricted 9Ai E8.5 Expression is in the endothelium.
TS13: primitive ventricle Present Regionally restricted 9Ai E8.5 Expression is in the endothelium.
Specimen 9Ai E9.0: embryonic day 9.0 (more )
Note: Confocal Z-projection. 16 somite pair embryo. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS14: common atrial chamber Present Regionally restricted 9Ai E9.0 Expression is in the endothelium.
TS14: primitive ventricle Present Regionally restricted 9Ai E9.0 Expression is in the endothelium.
Specimen 9Ai E9.5: embryonic day 9.5 (more )
Note: Confocal Z-projection. 22 somite pair embryo. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS15: heart atrium Present Regionally restricted 9Ai E9.5 Expression is in the endothelium.
TS15: primitive ventricle Present Regionally restricted 9Ai E9.5 Expression is in the endothelium.
Specimen 9B: embryonic day 10.5 (more )
Note: Confocal Z-projection. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS17: primitive ventricle Present Regionally restricted 9B Expression is in the endothelium.
Specimen 9C: embryonic day 10.5 (more )
Note: Confocal Z-projection. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS17: atrioventricular canal Present Regionally restricted 9C Expression is in the endothelium.
Specimen 9D Z-projection: embryonic day 10.5 (more )
Note: Confocal Z-projection. Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); blue - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 9D Z-Projection
Specimen 9D Single: embryonic day 10.5 (more )
Note: Single optical projection. Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); blue - Kit (assay MGI:6117874).`
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 9D Single
Specimen 9E: embryonic day 10.5 (more )
Note: Confocal Z-projection. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS17: heart endothelium Present 9E
Specimen 9F top: embryonic day 11.5 (more )
Note: Confocal Z-projection. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present 9F
Specimen 9F bottom left: embryonic day 11.5 (more )
Note: Confocal Z-projection. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present 9F bottom left
Specimen 9F bottom right: embryonic day 11.5 (more )
Note: Confocal Z-projection. Double labeled: red - Pecam1; white - Runx1 (assay MGI:6117870).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present 9F bottom right
Specimen 10A: embryonic day 9.5 (more )
Note: Confocal Z-projection. B: Magnified view of the boxed region in (A). Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS15: blood vessel Present Regionally restricted 10Bi , 10Ai Expression is in the endothelium.
TS15: vascular plexus Present Regionally restricted 10Ai , 10Bi Expression is in the endothelium of the cephalic vascular plexus.
Specimen 10C: embryonic day 10.0 (more )
Note: Confocal Z-projection. D: Magnified view of the boxed region in (C). Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS16: blood vessel Present Regionally restricted 10Ci , 10Di Expression is in the endothelium.
Specimen 10E: embryonic day 10.5 (more )
Note: Confocal Z-projection. Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: vascular plexus Present 10Ei , 10Fi Expression is in the endothelium of the cephalic vascular plexus.
Specimen 10G: embryonic day 10.5 (more )
Note: Confocal Z-projection. H: Magnified view of boxed region in (G). Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: vascular plexus Present Regionally restricted 10Hiv , 10Gi , 10Hi Expression is in the endothelium of the cephalic vascular plexus.
TS17: carotid artery Present Regionally restricted 10Gi , 10Hiv , 10Hi Expression is in the endothelium.
Specimen 11A: embryonic day 10.5 (more )
Note: Confocal Z-projection. B: Magnified view of the hematopoietic cluster shown in (A). Triple labeled: red - Pecam1; white - Runx1 (assay MGI:6117870); green - Kit (assay MGI:6117874).
Structure Level Pattern Image Note
TS17: blood vessel Present 11Bi , 11Ai Expression is in the endothelium.
TS17: dorsal aorta Present 11Bi , 11Ai Expression is in the endothelium.
TS17: intersomitic blood vessel Present 11Bi , 11Ai Expression is in the endothelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory