Gene Expression Data
Assay Details
Assay
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Reference:
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J:87368
Raetzman LT, et al., Developmental regulation of Notch signaling genes in the embryonic pituitary: Prop1 deficiency affects Notch2 expression. Dev Biol. 2004 Jan 15;265(2):329-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6117449
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Gene symbol:
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Notch3
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Gene name:
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notch 3
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Probe:
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Notch3 probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1I:
embryonic day 12.5
(more )
Specimen
1I:
(close )
Genetic Background:
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HsdOla:Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: adenohypophysis pars anterior |
Present |
Widespread |
1I
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Expression enriched in progenitor cells. Authors report expression in most progenitor cells at 12.5 dpc.
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Specimen
1J:
embryonic day 14.5
(more )
Specimen
1J:
(close )
Genetic Background:
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HsdOla:Swiss
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3A:
embryonic day 12.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: pituitary gland |
Present |
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3A
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Authors report similar levels of expression in pituitaries of each genotype.
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Specimen
3B:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Prop1df/Prop1df
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: pituitary gland |
Present |
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3B
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Authors report similar levels of expression in pituitaries of each genotype.
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