Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:87368
Raetzman LT, et al., Developmental regulation of Notch signaling genes in the embryonic pituitary: Prop1 deficiency affects Notch2 expression. Dev Biol. 2004 Jan 15;265(2):329-40
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6117442
|
|
Gene symbol:
|
Notch2
|
|
Gene name:
|
notch 2
|
|
|
Results
|
Specimen
2A:
embryonic day 12.5
(more  )
Specimen
2A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Prop1df/Prop1df
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: adenohypophysis |
Trace |
|
2B
|
Dramatically decreased expression in mutant pituitary.
|
| TS20: head mesenchyme |
Present |
Regionally restricted |
2B
|
Authors report expression remained unchanged in the surrounding mesenchyme relative to wild-type littermates.
|
Specimen
2C:
embryonic day 14.5
(more )
Specimen
2C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Prop1df/Prop1df
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: adenohypophysis |
Absent |
|
2D
|
Expression completely absent from mutant pituitary.
|
Specimen
2E:
embryonic day 16.5
(more )
Specimen
2E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
2F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Prop1df/Prop1df
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4 Wt Notch2:
embryonic day 18.5
(more )
Specimen
4 Wt Notch2:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools