Probe:	Myod cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present		3A , 3A inset	Expression first detected in branchial arches at approximately E9.75.
TS16: somite	Present		3A	Expression first detected in interlimb somites at E9.5-E9.75, initiated in a cranio-caudal fashion, occuring slightly later in occipito-cervical somites. Weak expression in somites anterior to forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	3B	Expression in developing facial musculature (e.g. extraocular muscle precursors).
TS17: 1st branchial arch	Present		3B
TS17: forelimb bud	Present		3B	Expression first detected in limb buds at E10.5
TS17: hindlimb bud	Present		3B	Expression first detected in limb buds at E10.5
TS17: somite	Present		3B

Structure	Level	Pattern	Image	Note
TS19: face	Present	Regionally restricted	3C	Expression in musculature of face.
TS19: branchial arch	Present		3C
TS19: forelimb bud	Present		3C
TS19: hindlimb bud	Present		3C
TS19: somite	Present		3C

Structure	Level	Pattern	Image	Note
TS20: myotome	Present	Regionally restricted	3D	Expression in myotomal muscle.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Absent		3E inset , 3E	Authors report expression eessentially eliminated in mandibular arch.
TS16: somite	Present		3E	Authors report a small reduction in expression in mutants, in a comparable number of somites to that of wild type. Weak expression in somites anterior to forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Ambiguous		3F	Expression dramatically lower than wild type in developing facial musculature (e.g. extraocular muscle precursors).
TS17: 1st branchial arch	Trace		3F	Expression barely detectable in mandibular arch. Authors report overstaining revealed traces of transcripts.
TS17: forelimb bud	Weak	Regionally restricted	3F	Authors report expression in limb buds was delayed in mutant embryos. Pronounced decrease in expression in muscle precursors of forelimb bud.
TS17: hindlimb bud	Ambiguous		3F
TS17: somite	Present		3F	Authors report a small reduction in expression in mutant embryos, with patterning resembling wild-type.
TS17: myotome	Present	Regionally restricted	3F	Authors report no specific defect in expression in muscle precursors subjacent to dermomyotomal lips or in young myocytes of epaxial and hypaxial myotome.

Structure	Level	Pattern	Image	Note
TS19: face	Present	Regionally restricted	3G	Expression in musculature of face remained lower in mutants embryos.
TS19: branchial arch	Present		3G	Authors report expression largely recovered in brahcnial arches and forelimb buds.
TS19: forelimb bud	Present		3G	Authors report expression largely recovered in brahcnial arches and forelimb buds.
TS19: hindlimb bud	Weak		3G	Expression remained significantly lower in mutant than in wild-type embryos.v
TS19: somite	Present		3G	Authors report a small reduction in expression in mutant embryos, with patterning resembling wild-type.
TS19: myotome	Present	Regionally restricted	3G	Authors report no specific defect in expression in muscle precursors subjacent to dermomyotomal lips or in young myocytes of epaxial and hypaxial myotome.

Structure	Level	Pattern	Image	Note
TS20: myotome	Present	Regionally restricted	3H	Authors report no difference in expression levels in myotomal muscle populations between wild-type and mutant embryos.

Structure	Level	Pattern	Image	Note
TS17: myotome	Ambiguous			Authors report expression in myotomes was delayed.

Structure	Level	Pattern	Image	Note
TS17: myotome	Ambiguous			Authors report expression in myotomes was delayed.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	6A	Authors report normal expression throughout mediolateral extent of myotome.v
TS19: branchial arch	Present		6A	Authors state expression was not affected by the Myf5 mutation.
TS19: limb bud	Present		6A	Authors state expression was not affected by the Myf5 mutation.

Structure	Level	Pattern	Image	Note
TS19: myotome	Weak	Regionally restricted	6B	Weak expression in ventrolateral myotomes of interlimb somites.
TS19: branchial arch	Present		6B	Authors state expression was not affected by the Myf5 mutation.
TS19: limb bud	Present		6B	Authors state expression was not affected by the Myf5 mutation.

Structure	Level	Pattern	Image	Note
TS19: myotome	Weak	Regionally restricted	6C	Weak expression in ventrolateral myotomes of interlimb somites.
TS19: branchial arch	Present		6C	Authors state expression was not affected by the Myf5 mutation.
TS19: limb bud	Present		6C	Authors state expression was not affected by the Myf5 mutation.

Structure	Level	Pattern	Image	Note
TS20: myotome	Present		6D

Structure	Level	Pattern	Image	Note
TS20: myotome	Present		6E	Normal expression levels in myotomal muscles.
TS20: trunk musculature	Present	Regionally restricted	6E	Authors report disorganization of trunk musculature as the result of rib defects.

Structure	Level	Pattern	Image	Note
TS20: myotome	Present		6F	Normal expression levels in myotomal muscles.
TS20: trunk musculature	Present	Regionally restricted	6F	Authors report disorganization of trunk musculature as the result of rib defects.