Xist RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:22243 Dolci S, et al., Expression of the Xist gene in urogenital ridges of midgestation male embryos. Biochem Biophys Res Commun. 1994 Nov 30;205(1):334-40
Assay type:	RT-PCR
MGI Accession ID:	MGI:6115632
Gene symbol:	Xist
Gene name:	inactive X specific transcripts

Probe:

Xist-pI, Xist-pJ

Results

Image: 2B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	309 bp	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Lane 1	Mol. Wt. Marker Lane
1	Cultured cells: no data stored
2	P7	TS28: spermatogonium	Present (a)	0.3 µg; total RNA	Not Specified		Male
3	P	TS28: spermatocyte	Present	1 µg; total RNA	Not Specified		Male	middle-late pachytene spermatocytes
4	Control
5	P	TS28: spermatid	Present (b)	1 µg; total RNA	Not Specified		Male	round spermatids
6	Control
7	P	TS28: spermatid	Absent	1 µg	Not Specified		Male	cytoplasmic RNA from round spermatids
8	P	TS28: spermatid	Absent	0.1 µg; poly-A+ RNA	Not Specified		Male	round spermatids
9	E12.5	TS20: ovary	Present	1 µg; total RNA	Not Specified		Female

Notes:
(a) Signal was detectable in spermatogonia using a higher number of cycles for RT-PCR amplification.
(b) The highest intensity of expression was noted in total cellular RNA preparations from round spermatids.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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