Probe:	Id4 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent		1_E6.5 lower right
TS9: extraembryonic component	Absent		1_E6.5 lower right

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		2G/H
TS11: extraembryonic component	Absent		2G/H

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present	Regionally restricted	3Q	Expressed in the ventral one-fourths near the lumen. Scattered exprression in the alar plate away from the lumen.
TS19: neural tube lateral wall	Present	Regionally restricted	3Q	Expressed in the ventral portion of the intermediate zone, in the presumptive motor neurons. Scattered exprression in the alar plate away from the lumen.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present		3R
TS19: midbrain	Present		3R
TS19: telencephalon	Present	Regionally restricted	3R	Expressed in the entire telencephalic vesicles, except the region where Id1, 2, and 3 are expressed.

Structure	Level	Pattern	Image	Note
TS19: hindbrain lateral wall	Present	Regionally restricted	3S	Expressed in the presumptive motor neurons.

Structure	Level	Pattern	Image	Note
TS19: myelencephalon	Present		3T

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		4E
TS20: midbrain	Present		4E
TS20: telencephalon	Present	Regionally restricted	4E	Expressed in the medial side of the telencephalic vesicles, located laterally to the third ventricle.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	4J	Expressed in many postmitotic nuclei.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	4O	Expressed in many postmitotic nuclei.
TS22: thalamus mantle layer	Present		4O
TS22: thalamus marginal layer	Present		4O
TS22: cerebral cortex ventricular layer	Present		4O
TS22: cortical plate	Present		4O
TS22: cerebral cortex intermediate zone	Absent		4O
TS22: ganglionic eminence	Present	Regionally restricted	4O	Expressed in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS24: brainstem nucleus	Present		4S
TS24: thalamic nucleus	Present		4S
TS24: hypothalamus	Present		4S	Expressed in nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellum layer	Present	Regionally restricted	6D	Expressed in presumptive interneurons adjacent to the Purkinje neurons located between the molecular and granular layers.

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present	Regionally restricted	6I	Expressed in the central region.
TS19: otocyst	Present		6I

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		6K	Expressed in the large cells (i.e., presumptive neurons).

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	7E	More concentrated in the inner side of the presumptive neural layer.
TS22: lens	Present	Scattered	7E

Structure	Level	Pattern	Image	Note
TS19: nasal pit epithelium	Present	Punctate	7J
TS19: nasal mesenchyme	Present		7J	Expressed in the area surrounding the nasal placode.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	7O	Expressed in the more mature postmitotic olfactory neuroepithelium, sandwiched between the two Id1, 2, 3-expressing layers.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: telencephalic vesicle	Present	Scattered

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Weak
TS17: acoustic ganglion	Present
TS17: facial ganglion	Present
TS17: glossopharyngeal ganglion	Present
TS17: trigeminal ganglion	Present
TS17: vagus ganglion	Present
TS17: optic cup inner layer	Present

Structure	Level	Pattern	Image	Note
TS19: infundibular recess of 3rd ventricle	Present
TS19: Rathke's pouch	Absent

Structure	Level	Pattern	Image	Note
TS20: vestibular ganglion	Present
TS20: cochlear ganglion	Absent

Structure	Level	Pattern	Image	Note
TS22: spinal cord gray matter	Present	Regionally restricted		Expressed in the pars ventralis, and in the future substantia gelatinosa.
TS22: spinal cord ventral horn	Present	Regionally restricted		Expressed in the motor neuron column.
TS22: cerebellum	Weak	Scattered

Structure	Level	Pattern	Image	Note
TS24: midbrain tegmentum	Present	Regionally restricted		Expressed in Raphe dorsalis.
TS24: pretectum	Present	Regionally restricted		Expressed in pretectal nucleus.
TS24: neural retina	Present
TS24: sympathetic ganglion	Present
TS24: lens	Present	Scattered
TS24: pigmented retinal epithelium	Absent