Gene Expression Data
Assay Details
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Assay
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Reference:
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J:126306
Nural HF, et al., The Slit receptor Robo1 is predominantly expressed via the Dutt1 alternative promoter in pioneer neurons in the embryonic mouse brain and spinal cord. Gene Expr Patterns. 2007 Oct;7(8):837-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5927615
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Gene symbol:
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Robo1
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Gene name:
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roundabout guidance receptor 1
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Probe:
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Dutt1 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2B:
embryonic day 10.5
(more  )
Specimen
2B:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: diencephalon |
Present |
Regionally restricted |
2B
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Expression extends in patches from the base of the optic stalk through ventral thalamus.
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| TS17: optic stalk |
Present |
Regionally restricted |
2B
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Expression extends in patches from the base of the optic stalk through ventral thalamus.
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| TS17: forebrain |
Present |
Regionally restricted |
2B
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Expression is in the posterior forebrain.
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| TS17: midbrain |
Present |
Regionally restricted |
2B
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Expression is in the anterior midbrain.
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| TS17: hindbrain |
Very strong |
Regionally restricted |
2B
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Expression is in a lateral band and in additional discrete stripes of cells dorsally.
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| TS17: rhombomere 4 |
Very strong |
Regionally restricted |
2B
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Expression is in a ventral block.
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Specimen
3A:
embryonic day 10.5
(more )
Note: Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
Specimen
3A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: diencephalon mantle layer |
Present |
Regionally restricted |
3B
,
3A
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Expression is in the mantle layer of the ventral thalamus in neurons and in the deeper neuroepithelial layer
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Specimen
4A:
embryonic day 10.5
(more )
Note: Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
Specimen
4A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain marginal layer |
Present |
Regionally restricted |
4D
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4B
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4A
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Expression is in the marginal zone of the dorsal half of the hindbrain.
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| TS17: hindbrain ventricular layer |
Present |
Regionally restricted |
4C
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4B
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4A
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4D
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Expression is in the ventral half of the ventricular zone in the neuroepithelium.
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| TS17: hindbrain |
Present |
Regionally restricted |
4C
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4A
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4D
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4B
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Expression is in the neurons along the margin of hindbrain.
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| TS17: hindbrain lateral wall |
Present |
Regionally restricted |
4D
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4A
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4B
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4C
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Expression is medially in the area of the hindbrain motor neurons.
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Specimen
5A:
embryonic day 10.5
(more )
Note: Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
Specimen
5A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
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Specimen
7A:
embryonic day 10.5
(more )
Note: Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
Specimen
7A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
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Expression is in both the neuroepithelium and in the mantle layer of neurons.
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| TS17: midbrain mantle layer |
Present |
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Expression is in both the neuroepithelium and in the mantle layer of neurons.
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