Gene Expression Data
Assay Details
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Assay
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Reference:
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J:209070
Glaser A, et al., Tbx4 interacts with the short stature homeobox gene Shox2 in limb development. Dev Dyn. 2014 May;243(5):629-39
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5912540
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Gene symbol:
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Tbx4
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Gene name:
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T-box 4
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Probe:
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Tbx4 cDNA4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2Af:
embryonic day 9.5
(more  )
Specimen
2Af:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2Ag:
embryonic day 10.5
(more )
Specimen
2Ag:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
2Ag
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Expressed in a discrete localized region of the proximal limb bud.
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| TS17: hindlimb bud |
Strong |
Widespread |
2Ag
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Expressed in the entire hindlimb bud.
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Specimen
2Ah:
embryonic day 11.5
(more )
Specimen
2Ah:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Widespread |
2Ah
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Decreased expression. Expressed more diffusely in the distal part.
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| TS19: hindlimb bud |
Present |
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2Ah
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Expressed in the entire hindlimb bud.
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Specimen
2Ai:
embryonic day 12.5
(more )
Specimen
2Ai:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
2Ai
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Expression proceeds distally.
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| TS20: hindlimb |
Present |
Widespread |
2Ai
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Expressed in the entire hindlimb bud.
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Specimen
2Aj:
embryonic day 13.5
(more )
Specimen
2Aj:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb |
Present |
Regionally restricted |
2Aj
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Expression proceeds distally.
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| TS21: hindlimb |
Present |
Regionally restricted |
2Aj
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Expression is more distally restricted.
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Specimen
2Bb:
embryonic day 10.5
(more )
Specimen
2Bb:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
2Bb
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Expressed in the proximal region, overlaping with the broader of Shox2 expression domain.
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Specimen
2Bb':
embryonic day 10.5
(more )
Specimen
2Bb':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2Bb'
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Expression broadly overlaps with Shox2 throughout the proximal limb mesenchyme.
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Specimen
2Be:
embryonic day 11.5
(more )
Specimen
2Be:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
2Be
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Expressed in a region corresponding to a Col2a1-positive cartilaginous element of the dorsal forelimb. Expression patterns of Shox2 and Tbx4 are mutually exclusive.
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Specimen
2Be':
embryonic day 11.5
(more )
Specimen
2Be':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2Be'
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Expression broadly overlaps with Shox2 throughout the proximal limb mesenchyme.
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Specimen
2Bh:
embryonic day 12.5
(more )
Specimen
2Bh:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb pre-cartilage condensation |
Present |
Regionally restricted |
2Bh
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Expressed in a region corresponding to a Col2a1-positive cartilaginous element of the dorsal forelimb. Expression patterns of Shox2 and Tbx4 are mutually exclusive.
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Specimen
2Bh':
embryonic day 12.5
(more )
Specimen
2Bh':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb mesenchyme |
Present |
Regionally restricted |
2Bh'
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Expressed in areas surrounding the proximal cartilaginous elements.
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Specimen
3Aa:
embryonic day 10.5
(more )
Specimen
3Aa:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3Ab:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * CD-1
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Mutant Allele(s):
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Shox2tm1Acgg/Shox2tm1Acgg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
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3Ab
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No altered expression compared with the wild-type.
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Specimen
3Ac:
embryonic day 11.5
(more )
Specimen
3Ac:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3Ad:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * CD-1
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Mutant Allele(s):
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Shox2tm1Acgg/Shox2tm1Acgg
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
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3Ad
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Increased expression in the specific dorsal expressing region, compared with the wild-type.
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Specimen
3Ae:
embryonic day 12.5
(more )
Specimen
3Ae:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3Af:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * CD-1
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Mutant Allele(s):
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Shox2tm1Acgg/Shox2tm1Acgg
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
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3Af
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Increased expression in the specific dorsal expressing region, compared with the wild-type.
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Specimen
3Ag:
embryonic day 13.5
(more )
Specimen
3Ag:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3Ah:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * CD-1
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Mutant Allele(s):
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Shox2tm1Acgg/Shox2tm1Acgg
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb |
Present |
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3Ah
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Increased expression in the specific dorsal expressing region, compared with the wild-type.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * CD-1
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Mutant Allele(s):
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Shox2tm1Acgg/Shox2tm1Acgg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
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No altered expression compared with the wild-type.
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Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * CD-1
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Mutant Allele(s):
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Shox2tm1Acgg/Shox2tm1Acgg
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
|
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No altered expression compared with the wild-type.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * CD-1
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|
Mutant Allele(s):
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Shox2tm1Acgg/Shox2tm1Acgg
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Age:
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embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
|
|
No altered expression compared with the wild-type.
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Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
|
whole mount
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|
Fixation:
|
4% Paraformaldehyde
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * CD-1
|
|
Mutant Allele(s):
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Shox2tm1Acgg/Shox2tm1Acgg
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|
Age:
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embryonic day 13.5
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Sex:
|
Not Specified
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Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindlimb |
Present |
|
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No altered expression compared with the wild-type.
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